Itokazu M, Tanaka S
Nihon Seikeigeka Gakkai Zasshi. 1985 Dec;59(12):1089-96.
FITC-conjugated lectins, Con-A, DBA, GS-I, GS-II, PNA, MPA, RCA-I, SBA, UEA-I, WGA were used for demonstration of lectin bindings of human synovial lining cells, obtained from the patients with rheumatoid arthritis (RA), osteoarthritis (OA), aseptic necrosis (AN), and traumatic injury (TI). In the RA samples, GS-I binding to the cytoplasmic sites was predominantly noted and moderate SBA and MPA bindings were observed. However, PNA was not significant. In the OA samples, predominant binding was found in GS-I and SBA lectins, moderate binding in MPA and PNA. In the AN samples, binding was predominant in MPA, moderate in GS-I, SBA and PNA. After neuraminidase treatment the intensity of fluorescence increased significantly with PNA and moderately with SBA in the RA samples. These results suggested that the inflammatory lining cells produce galactose group and the content of neuraminic acids in the synovial membranes of the RA appears to be greater than in those of other diseases.
使用异硫氰酸荧光素（FITC）偶联的凝集素，如刀豆球蛋白A（Con-A）、麦芽凝集素（DBA）、大豆凝集素I（GS-I）、大豆凝集素II（GS-II）、花生凝集素（PNA）、麦胚凝集素（MPA）、蓖麻凝集素I（RCA-I）、大豆凝集素（SBA）、荆豆凝集素I（UEA-I）、小麦胚凝集素（WGA），来检测从类风湿性关节炎（RA）、骨关节炎（OA）、无菌性坏死（AN）和创伤性损伤（TI）患者获取的人滑膜衬里细胞的凝集素结合情况。在RA样本中，主要观察到GS-I与细胞质位点的结合，同时观察到中等程度的SBA和MPA结合。然而，PNA结合不显著。在OA样本中，主要在GS-I和SBA凝集素中发现结合，MPA和PNA中为中等程度结合。在AN样本中，MPA结合为主，GS-I、SBA和PNA为中等程度结合。在RA样本中，经神经氨酸酶处理后，PNA的荧光强度显著增加，SBA的荧光强度适度增加。这些结果表明，炎症衬里细胞产生半乳糖基团，RA滑膜中神经氨酸的含量似乎高于其他疾病。
