使用大鼠和小鼠肝细胞的肝细胞原代培养/DNA修复试验中多种肼衍生物的遗传毒性。
Genotoxicity of a variety of hydrazine derivatives in the hepatocyte primary culture/DNA repair test using rat and mouse hepatocytes.
作者信息
Mori H, Sugie S, Yoshimi N, Iwata H, Nishikawa A, Matsukubo K, Shimizu H, Hirono I
机构信息
Department of Pathology, Gifu University School of Medicine.
出版信息
Jpn J Cancer Res. 1988 Feb;79(2):204-11. doi: 10.1111/j.1349-7006.1988.tb01578.x.
The genotoxicity of a variety of hydrazine derivatives was examined in the DNA-repair test on rat or mouse hepatocytes. Out of 32 hydrazine derivatives, 6 chemicals, i.e., N'-acetyl-4-(hydroxymethyl)phenylhydrazine, 1,2-dimethylhydrazine.2HCl, 1-hydrazinophthalazine.HCl, methylhydrazine.sulfate, p,p'-oxybisbenzene disulfonylhydrazide and phenylhydrazine.HCl, elicited positive DNA repair responses in the test on rat hepatocytes. In the test on mouse hepatocytes, 4 more hydrazine derivatives, i.e., 1,1-dimethylhydrazine, hydrazine hydrate, hydrazine sulfate and 2-methyl-4-chlorophenoxyacetic acid hydrazide.HCl also generated positive responses, in addition to the 6 positive compounds in the rat assay. These results suggest that mouse hepatocytes are more susceptible to the genotoxicity of hydrazine derivatives, and that the species differences in genotoxicity appear to be in agreement with the in vivo carcinogenicity of these agents.
在大鼠或小鼠肝细胞的DNA修复试验中检测了多种肼衍生物的遗传毒性。在32种肼衍生物中，6种化学物质，即N'-乙酰基-4-（羟甲基）苯肼、1,2-二甲基肼·2HCl、1-肼基酞嗪·HCl、甲基肼·硫酸盐、对，对'-氧双苯二磺酰肼和苯肼·HCl，在大鼠肝细胞试验中引发了阳性DNA修复反应。在小鼠肝细胞试验中，除了大鼠试验中的6种阳性化合物外，另外4种肼衍生物，即1,1-二甲基肼、水合肼、硫酸肼和2-甲基-4-氯苯氧基乙酸酰肼·HCl也产生了阳性反应。这些结果表明，小鼠肝细胞对肼衍生物的遗传毒性更敏感，并且遗传毒性的种属差异似乎与这些物质的体内致癌性一致。
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