Mori H, Sugie S, Yoshimi N, Kinouchi T, Ohnishi Y
Mutat Res. 1987 Feb;190(2):159-67. doi: 10.1016/0165-7992(87)90049-2.
Genotoxicity of a variety of nitroarenes and other compounds was examined in DNA-repair tests with rat or mouse hepatocytes. Out of 15 nitroarenes tested, 9 compounds, i.e., 1-nitropyrene, 1,3-dinitropyrene, 1,6-dinitropyrene, 1,8-dinitropyrene, 1-nitro-3-acetoxypyrene, 3-nitrofluoranthene, 2-nitrofluorene, 2,7-di-nitrofluorene and 5-nitroacenaphthene elicited positive response of DNA repair in the tests with rat and mouse hepatocytes. Among the positive chemicals, the DNA-repair level of the 3 dinitropyrene isomers was much higher than other nitroarenes. The results indicate that a number of nitroarenes are metabolically activated in the primary culture of rodent hepatocytes, and suggest potential carcinogenicity of 1-nitropyrene and 1-nitro-3-acetoxypyrene the carcinogenicity of which is either not clear or unknown. Of the other nitro compounds, 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide as well as 4-nitroquinoline 1-oxide were clearly genotoxic in the assays with hepatocytes of both species. However, 5-nitro-2-furaldehyde semicarbazone was negative in both assays with hepatocytes of 2 species.
在大鼠或小鼠肝细胞的DNA修复试验中检测了多种硝基芳烃和其他化合物的遗传毒性。在测试的15种硝基芳烃中，9种化合物，即1-硝基芘、1,3-二硝基芘、1,6-二硝基芘、1,8-二硝基芘、1-硝基-3-乙酰氧基芘、3-硝基荧蒽、2-硝基芴、2,7-二硝基芴和5-硝基苊在大鼠和小鼠肝细胞试验中引发了DNA修复的阳性反应。在阳性化学物质中，3种二硝基芘异构体的DNA修复水平远高于其他硝基芳烃。结果表明，许多硝基芳烃在啮齿动物肝细胞原代培养中被代谢激活，并提示1-硝基芘和1-硝基-3-乙酰氧基芘具有潜在致癌性，其致癌性尚不明确或未知。在其他硝基化合物中，2-(2-呋喃基)-3-(5-硝基-2-呋喃基)丙烯酰胺以及4-硝基喹啉1-氧化物在两种动物肝细胞试验中均具有明显的遗传毒性。然而，5-硝基-2-糠醛半卡巴腙在两种动物肝细胞试验中均为阴性。
