Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Int J Biol Macromol. 2019 Oct 1;138:70-78. doi: 10.1016/j.ijbiomac.2019.07.073. Epub 2019 Jul 12.
Avian Leukosis Virus Subgroup J (ALV-J) is an oncogenic retrovirus, mainly spread by vertical and horizontal transmission, which have caused severe losses in world poultry industry. Sargassum fusiforme polysaccharide (SFP), a marine algae sulfated polysaccharide, has attracted more attention due to its variously biological activities. In this study, the anti-ALV-J property of SFP was assessed in vivo and in vitro. The results demonstrated that different Mw of SFPs showed virustatic activity to ALV-J in vitro by combining with the virus when ALV-J adsorbed onto the host cells. When treated with SFPs, the ALV-J gene and protein expression reduced clearly and SFP-3 (Molecular weight 9 kDa) had the best antiviral effect. Results in vivo showed that the immunosuppression of the ALV-J infected chickens were relieved by SFP-3. Moreover, SFP-3 obviously inhibit the viral shedding and alleviated the organs damage caused by ALV-J. This study offered a new method for ALV-J treatment and enriched the potential application of SFP.
禽白血病病毒 J 亚群（ALV-J）是一种致癌性逆转录病毒，主要通过垂直和水平传播，给世界家禽业造成了严重损失。马尾藻多糖（SFP）是一种海洋藻类硫酸多糖，由于其多种生物活性而受到越来越多的关注。本研究在体内和体外评估了 SFP 的抗 ALV-J 特性。结果表明，不同分子量的 SFPs 在 ALV-J 吸附到宿主细胞时与病毒结合，表现出体外的抗病毒活性。用 SFPs 处理后，ALV-J 基因和蛋白表达明显减少，SFP-3（分子量 9 kDa）的抗病毒效果最好。体内结果表明，SFP-3 缓解了 ALV-J 感染鸡的免疫抑制。此外，SFP-3 明显抑制病毒脱落，减轻了 ALV-J 引起的器官损伤。本研究为 ALV-J 的治疗提供了一种新方法，丰富了 SFP 的潜在应用。
