Department of Physiology, Institute of Bioscience of the University of São Paulo, São Paulo, Brazil.
Department of Physiology, Institute of Bioscience of the University of São Paulo, São Paulo, Brazil.
Curr Opin Microbiol. 2019 Dec;52:110-115. doi: 10.1016/j.mib.2019.06.003. Epub 2019 Jul 12.
The outcome of Leishmania infection depends on the parasite species and the host immune response. Virulence factors have been extensively studied over the years in an effort to find efficient vaccines and/or treatments for Leishmania infection. Arginase activity in Leishmania has been described as an essential player for the polyamines pathway, impacting parasite replication and infectivity. Considering previous studies showing that the absence of arginase activity leads to low infectivity of Leishmania amazonensis, we reanalyzed transcriptomic data comparing both promastigotes and axenic amastigotes from L. amazonensis wild type (La-WT) and L. amazonensis arginase knockout (La-arg) backgrounds. The analysis produced a new compilation of modulated transcripts that indicated the role of arginase not only in the polyamines pathway but also in the modulation of virulence factors involved in parasite recognition, growth and differentiation.
利什曼原虫感染的结果取决于寄生虫种类和宿主免疫反应。多年来，人们一直在广泛研究毒力因子，以期找到针对利什曼原虫感染的有效疫苗和/或治疗方法。利什曼原虫中的精氨酸酶活性被描述为多胺途径的一个重要参与者，影响寄生虫的复制和感染力。鉴于先前的研究表明，缺乏精氨酸酶活性会导致莱什曼原虫亚马逊亚种（L. amazonensis）的感染力降低，我们重新分析了比较 L. amazonensis 野生型（La-WT）和 L. amazonensis 精氨酸酶敲除（La-arg）背景下的前鞭毛体和无细胞内阿米巴型的转录组数据。该分析产生了一个新的调节转录物的汇编，表明精氨酸酶的作用不仅在多胺途径中，而且在寄生虫识别、生长和分化相关的毒力因子的调节中也发挥作用。
