Department of Radiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang Road West, Guangzhou, 510120, China.
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry/KLGHEI of Environment and Energy Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
Anal Chim Acta. 2019 Oct 24;1077:67-86. doi: 10.1016/j.aca.2019.05.054. Epub 2019 May 25.
The determination of endogenous substances is of great significance for obtaining important biotic information such as biological components, metabolic pathways and disease biomarkers in different living organisms (e.g. plants, insects, animals and humans). However, due to the complex matrix and the trace concentrations of target analytes, the sample preparation procedure is an essential step before the analytes of interest are introduced into a detection instrument. Solid-phase microextraction (SPME), an emerging sample preparation technique that integrates sampling, extraction, concentration, and sample introduction into one step, has gained wide acceptance in various research fields, including in the determination of endogenous compounds. In this review, recent developments and applications of SPME for the determination of endogenous substances over the past five years are summarized. Several aspects, including the design of SPME devices (sampling configuration and coating), applications (in vitro and in vivo sampling), and coupling with emerging instruments (comprehensive two-dimensional gas chromatography (GC × GC), ambient mass spectrometry (AMS) and surface enhanced Raman scattering (SERS)) are involved. Finally, the challenges and opportunities of SPME methods in endogenous substances analysis are also discussed.
内源性物质的测定对于获取不同生物体（如植物、昆虫、动物和人类）中的生物成分、代谢途径和疾病生物标志物等重要生物信息具有重要意义。然而，由于复杂的基质和痕量浓度的目标分析物，在将感兴趣的分析物引入检测仪器之前，样品制备程序是必不可少的步骤。固相微萃取（SPME）是一种新兴的样品制备技术，它将采样、萃取、浓缩和进样集于一体，在包括内源性化合物测定在内的各个研究领域得到了广泛的认可。本综述总结了过去五年内 SPME 用于内源性物质测定的最新发展和应用。涉及 SPME 装置的设计（采样配置和涂层）、应用（体外和体内采样）以及与新兴仪器的耦合（全二维气相色谱（GC×GC）、环境质谱（AMS）和表面增强拉曼散射（SERS））等几个方面。最后，还讨论了 SPME 方法在分析内源性物质方面的挑战和机遇。
