Sefidgar Seyed Mersad, Ahmadi-Hamedani Mahmood, Jebelli Javan Ashkan, Narenji Sani Reza, Javaheri Vayghan Abbas
Student Research Committee, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Avicenna J Phytomed. 2019 Jul-Aug;9(4):347-361.
Chronic hyperglycemia and overproduction of reactive oxygen species (ROS) are strong predictors of the development of reproductive complications of diabetes. The present study was conducted to determine the effects of crocin on biochemical parameters, oxidative stress, and sperm characteristics as well as testes histopathology in diabetic rats.
Twenty-four rats were divided into the four groups as follows: control, untreated diabetic and two crocin (40 and 60 mg/kg/day)-treated diabetic groups. Diabetes was induced by injection of a single dose of streptozotocin (STZ, 60 mg/kg). Administration of crocin (intraperitoneally) was started three days after STZ injection and was continued until the 28 day. At the end of the experiment, rats were anesthetized after weighing. Blood samples and epididymal sperm were subsequently collected to measure biochemical parameters (glucose and lipid profile), total oxidant and antioxidant status (TOS and TAS, respectively), oxidative stress index (OSI), and sperm characteristics (count, motility, and viability); also, testes were dissected out for histopathology examination.
Our result indicated that blood glucose, cholesterol, triglyceride, LDL cholesterol levels, as well as TOS, and OSI decreased, but body weight, sperm counts, motility and viability, as well as TAS and HDL levels increased significantly in the crocin-treated diabetic rats (P˂0.05). In testis sections from diabetic rats treated with crocin (40 and 60 mg/kg), seminiferous tubules exhibited normal shape and restoration of testis architecture was observed.
Administration of crocin in the present study, ameliorated blood glucose, lipid abnormalities, oxidative stress, sperm characteristics and testis damage in STZ-diabetic rats.
慢性高血糖和活性氧（ROS）的过度产生是糖尿病生殖并发症发生的有力预测指标。本研究旨在确定西红花苷对糖尿病大鼠生化参数、氧化应激、精子特征以及睾丸组织病理学的影响。
将24只大鼠分为以下四组：对照组、未治疗的糖尿病组以及两个用西红花苷（40和60毫克/千克/天）治疗的糖尿病组。通过注射单剂量链脲佐菌素（STZ，60毫克/千克）诱导糖尿病。在注射STZ三天后开始腹腔注射西红花苷，并持续至第28天。实验结束时，称重后对大鼠进行麻醉。随后采集血样和附睾精子，以测量生化参数（血糖和血脂谱）、总氧化剂和抗氧化剂状态（分别为TOS和TAS）、氧化应激指数（OSI）以及精子特征（数量、活力和存活率）；同时，取出睾丸进行组织病理学检查。
我们的结果表明，在用西红花苷治疗的糖尿病大鼠中，血糖、胆固醇、甘油三酯、低密度脂蛋白胆固醇水平以及TOS和OSI降低，但体重、精子数量、活力和存活率以及TAS和高密度脂蛋白水平显著升高（P˂0.05）。在用西红花苷（40和60毫克/千克）治疗的糖尿病大鼠的睾丸切片中，生精小管呈现正常形态，观察到睾丸结构得到恢复。
在本研究中，给予西红花苷改善了STZ诱导的糖尿病大鼠的血糖、脂质异常、氧化应激、精子特征和睾丸损伤。
