Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Department of Psychology, University of California, Berkeley, Berkeley, CA, USA.
Sci Adv. 2019 Jul 10;5(7):eaaw3108. doi: 10.1126/sciadv.aaw3108. eCollection 2019 Jul.
Neuromodulation plays a critical role in brain function in both health and disease, and new tools that capture neuromodulation with high spatial and temporal resolution are needed. Here, we introduce a synthetic catecholamine nanosensor with fluorescent emission in the near infrared range (1000-1300 nm), near infrared catecholamine nanosensor (nIRCat). We demonstrate that nIRCats can be used to measure electrically and optogenetically evoked dopamine release in brain tissue, revealing hotspots with a median size of 2 µm. We also demonstrated that nIRCats are compatible with dopamine pharmacology and show D2 autoreceptor modulation of evoked dopamine release, which varied as a function of initial release magnitude at different hotspots. Together, our data demonstrate that nIRCats and other nanosensors of this class can serve as versatile synthetic optical tools to monitor neuromodulatory neurotransmitter release with high spatial resolution.
神经调节在健康和疾病中的大脑功能中起着关键作用，需要新的工具来以高时空分辨率捕捉神经调节。在这里，我们引入了一种具有近红外发射（1000-1300nm）的合成儿茶酚胺纳米传感器，近红外儿茶酚胺纳米传感器（nIRCat）。我们证明，nIRCats 可用于测量脑组织中电和光遗传诱发的多巴胺释放，揭示中位数大小为 2 µm 的热点。我们还证明 nIRCats 与多巴胺药理学兼容，并显示出诱发多巴胺释放的 D2 自身受体调节，其在不同热点处随初始释放幅度的变化而变化。总之，我们的数据表明，nIRCats 和此类纳米传感器的其他变体可以作为多功能合成光学工具，以高空间分辨率监测神经调节神经递质的释放。
