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来自耐冷菌 Psychrobacter arcticus 273-4 的细胞壁相关多糖:分离、纯化和结构解析。

Cell-wall associated polysaccharide from the psychrotolerant bacterium Psychrobacter arcticus 273-4: isolation, purification and structural elucidation.

机构信息

Department of Chemical Sciences, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia, 80126, Naples, Italy.

出版信息

Extremophiles. 2020 Jan;24(1):63-70. doi: 10.1007/s00792-019-01113-8. Epub 2019 Jul 15.

Abstract

In this paper, the structure of the capsular polysaccharide isolated from the psychrotolerant bacterium Psychrobacter arcticus 273-4 is reported. The polymer was purified by gel filtration chromatography and the structure was elucidated by means of one- and two-dimensional NMR spectroscopy, in combination with chemical analyses. The polysaccharide consists of a trisaccharidic repeating unit containing two residues of glucose and a residue of a N,N-diacetyl-pseudaminic acid.

摘要

本文报道了从耐冷菌 Psychrobacter arcticus 273-4 中分离得到的荚膜多糖的结构。该聚合物通过凝胶过滤色谱法进行纯化,并通过一维和二维 NMR 光谱学结合化学分析来阐明其结构。该多糖由一个三糖重复单元组成,包含两个葡萄糖残基和一个 N,N-二乙酰基伪冬氨酸残基。

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