ACS Appl Mater Interfaces. 2019 Aug 7;11(31):27548-27557. doi: 10.1021/acsami.9b08047. Epub 2019 Jul 26.
The near-infrared fluorescent (NIRF) dye, IR780, is recognized as a promising theranostic agent and has been widely investigated for imaging, chemotherapeutic, and phototherapeutic applications. However, its poor photostability and nonselective toxicities toward both cancer and normal cells limit its biological applications. Herein, we introduce the use of GUMBOS (a group of uniform materials based on organic salts) developed through counter-anion exchange with IR780 and subsequent nanomaterials (nanoGUMBOS) formed by complexation with cyclodextrin (CD) for enhanced chemo/photothermal therapy. Such CD-based nanoGUMBOS display improved aqueous stability, photostability, and photothermal effects relative to traditional IR780. The examination of in vitro cytotoxicity reveals that CD-based nanoGUMBOS are selectively toxic toward cancer cells and exhibit synergistically enhanced cytotoxicity toward cancer cells upon NIR laser irradiation. Additionally, in vivo NIRF imaging demonstrated selective accumulation of these nanoGUMBOS within the tumor site, indicating tumor-targeting properties. Further in vivo therapeutic study of these CD-based nanoGUMBOS suggests excellent chemo/photothermal antitumor effects. Using these studies, we herein demonstrate a promising strategy, via conversion of IR780 into nanoGUMBOS, that can be used for improved theranostic cancer treatment.
近红外荧光（NIRF）染料 IR780 被认为是一种很有前途的治疗诊断试剂，已广泛应用于成像、化学治疗和光疗等领域。然而，其较差的光稳定性和对癌症和正常细胞的非选择性毒性限制了其生物应用。在此，我们介绍了使用通过与 IR780 进行反离子交换并随后与环糊精（CD）络合形成的纳米材料（nanoGUMBOS）来开发的 GUMBOS（一组基于盐的均匀材料），以增强化学/光热治疗效果。与传统的 IR780 相比，基于 CD 的 nanoGUMBOS 具有更好的水稳定性、光稳定性和光热效应。体外细胞毒性研究表明，基于 CD 的 nanoGUMBOS 对癌细胞具有选择性毒性，并在近红外激光照射下表现出协同增强的细胞毒性。此外，体内 NIRF 成像显示这些纳米 GUMBOS 选择性地积聚在肿瘤部位，表明具有肿瘤靶向特性。进一步的体内治疗研究表明，这些基于 CD 的 nanoGUMBOS 具有优异的化学/光热抗肿瘤效果。通过将 IR780 转化为 nanoGUMBOS，我们在此展示了一种有前途的策略，可用于改善癌症的治疗诊断效果。
