Marine College, Shandong University, Weihai 264209, PR China.
State key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3056-3061. doi: 10.1099/ijsem.0.003589.
A novel Gram-stain-negative, facultatively anaerobic, oxidase-negative, catalase-positive bacillus, designated WD2A32, was isolated from a marine solar saltern in the coast of Weihai, Shandong Province, PR China. Strain WD2A32 grew optimally at 37-40 °C (range, 20-45 °C) and pH 7.0-7.5 (range, 6.5-8.0) and was tolerant to 6-8 % (w/v) NaCl (range, 2-12 %). The 16S rDNA of strain WD2A32 was most similar (93.5 %) to that of DSM 9154 (the type species of the genus ), followed by DSM 25584 (92.4 %; the type species of the genus ). The similarities to the type strains of the genera and were less than 91.0 %. The draft genome sequence of strain WD2A32 contained 72 contigs (>507 bp) of 4 237 996 bp with a DNA G+C content of 65.5 mol%. The major polar lipids of strain WD2A32 were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and unidentified aminolipids; major cellular fatty acids were summed feature 8 (Cω7/Cω6) and C cyclo ω8; the major respiratory quinone was Q-10. On the basis of the results from chemotaxonomic, physiological and biochemical analyses, strain WD2A32 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. has been proposed. The type strain is WD2A32 (=KCTC 52888=MCCC 1H00201).
一株新型革兰氏阴性、兼性厌氧、氧化酶阴性、接触酶阳性的杆菌，被命名为 WD2A32，从中国山东省威海市沿海的一个海洋盐场中分离得到。菌株 WD2A32 在 37-40°C（范围，20-45°C）和 pH 7.0-7.5（范围，6.5-8.0）下生长最佳，耐受 6-8%（w/v）NaCl（范围，2-12%）。菌株 WD2A32 的 16S rDNA 与 DSM 9154（属的模式种）最为相似（93.5%），其次是 DSM 25584（92.4%；属的模式种）。与属的模式菌株的相似度均小于 91.0%。菌株 WD2A32 的基因组草图包含 72 个大于 507bp 的 contigs，大小为 4,237,996bp，GC 含量为 65.5mol%。菌株 WD2A32 的主要极性脂为磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和未鉴定的胺脂；主要细胞脂肪酸为 summed feature 8 (Cω7/Cω6) 和 C 环 ω8；主要呼吸醌为 Q-10。基于化学分类、生理生化分析的结果，菌株 WD2A32 代表了一个新型属的一个新型种，该属被提议命名为 gen. nov.，种名 nov.。模式菌株为 WD2A32（=KCTC 52888=MCCC 1H00201）。
