使用构巢曲霉amdS基因作为显性选择标记对产黄青霉进行转化。
Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker.
作者信息
Beri R K, Turner G
机构信息
Department of Microbiology, University of Bristol, UK.
出版信息
Curr Genet. 1987;11(8):639-41. doi: 10.1007/BF00393928.
PMID:3131026
Abstract
The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites.
摘要
构巢曲霉乙酰胺酶基因（amdS）已被用于以低频率转化产黄青霉。对多个转化体进行了测试，结果表明它们在有丝分裂过程中是稳定的。Southern印迹分析表明，转化DNA已整合到染色体DNA中，可能整合到了多个位点。
Zotero 插件