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使用构巢曲霉amdS基因作为显性选择标记对产黄青霉进行转化。

Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker.

作者信息

Beri R K, Turner G

机构信息

Department of Microbiology, University of Bristol, UK.

出版信息

Curr Genet. 1987;11(8):639-41. doi: 10.1007/BF00393928.

DOI:10.1007/BF00393928
PMID:3131026
Abstract

The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites.

摘要

构巢曲霉乙酰胺酶基因（amdS）已被用于以低频率转化产黄青霉。对多个转化体进行了测试，结果表明它们在有丝分裂过程中是稳定的。Southern印迹分析表明，转化DNA已整合到染色体DNA中，可能整合到了多个位点。

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Curr Genet. 1987;11(8):639-41. doi: 10.1007/BF00393928.
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本文引用的文献

1
Transformation by integration in Aspergillus nidulans.构巢曲霉中的整合转化
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2
Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations.构巢曲霉amdS基因基因组克隆的分离及其在结构和调控突变分析中的应用。
Mol Cell Biol. 1983 Aug;3(8):1430-9. doi: 10.1128/mcb.3.8.1430-1439.1983.
3
Transformation of Aspergillus nidulans by using a trpC plasmid.利用trpC质粒对构巢曲霉进行转化。
一款在生物技术领域具有前沿形象的复古模型。
Microorganisms. 2022 Mar 6;10(3):573. doi: 10.3390/microorganisms10030573.
4
Transformation of 212 and Expression of GFP and DsRED Coding Genes for Visualization of Plant-Biocontrol Agent Interaction.212的转化以及用于可视化植物与生物防治剂相互作用的绿色荧光蛋白（GFP）和红色荧光蛋白（DsRED）编码基因的表达
Front Microbiol. 2018 Jul 23;9:1653. doi: 10.3389/fmicb.2018.01653. eCollection 2018.
5
Displaying Candida antarctica lipase B on the cell surface of Aspergillus niger as a potential food-grade whole-cell catalyst.在黑曲霉细胞表面展示南极假丝酵母脂肪酶 B 作为一种有潜力的食品级全细胞催化剂。
J Ind Microbiol Biotechnol. 2014 Apr;41(4):711-20. doi: 10.1007/s10295-014-1410-y. Epub 2014 Feb 12.
6
Expression of the Aspergillus niger glucose oxidase gene in Penicillium nalgiovense.黑曲霉葡萄糖氧化酶基因在宛氏拟青霉中的表达。
World J Microbiol Biotechnol. 1995 May;11(3):322-5. doi: 10.1007/BF00367109.
7
amdSYM, a new dominant recyclable marker cassette for Saccharomyces cerevisiae.amdSYM，一种新型的酿酒酵母优势可回收标记盒。
FEMS Yeast Res. 2013 Feb;13(1):126-39. doi: 10.1111/1567-1364.12024. Epub 2012 Dec 17.
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Appl Microbiol Biotechnol. 1993 Jan;38(4):514-9. doi: 10.1007/BF00242947.
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10
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5
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