在高盐环境中蛋白质-DNA 相互作用的特异性：嗜盐古菌依赖氧化应激的蛋白质 hsRosR 复合物的结构研究。
Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.
机构信息
Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410510, Israel.
National Institute of Biotechnology in the Negev, Ben-Gurion University, Beer Sheva 8410510, Israel.
出版信息
Nucleic Acids Res. 2019 Sep 19;47(16):8860-8873. doi: 10.1093/nar/gkz604.
Interactions between proteins and DNA are crucial for all biological systems. Many studies have shown the dependence of protein-DNA interactions on the surrounding salt concentration. How these interactions are maintained in the hypersaline environments that halophiles inhabit remains puzzling. Towards solving this enigma, we identified the DNA motif recognized by the Halobactrium salinarum ROS-dependent transcription factor (hsRosR), determined the structure of several hsRosR-DNA complexes and investigated the DNA-binding process under extreme high-salt conditions. The picture that emerges from this work contributes to our understanding of the principles underlying the interplay between electrostatic interactions and salt-mediated protein-DNA interactions in an ionic environment characterized by molar salt concentrations.
蛋白质和 DNA 之间的相互作用对所有生物系统都至关重要。许多研究表明，蛋白质-DNA 相互作用依赖于周围盐浓度。然而，在嗜盐生物栖息的高盐环境中，这些相互作用是如何维持的仍然令人费解。为了解决这个谜团，我们确定了 Halobactrium salinarum 依赖 ROS 的转录因子（hsRosR）识别的 DNA 基序，确定了几个 hsRosR-DNA 复合物的结构，并在极端高盐条件下研究了 DNA 结合过程。这项工作所呈现的图景有助于我们理解在摩尔盐浓度为特征的离子环境中，静电相互作用和盐介导的蛋白质-DNA 相互作用之间相互作用的原理。
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