聚合物宏观纳米盘的磁定向使基于残余偶极耦合的高分辨率结构研究成为可能 通过 NMR 光谱。
Magnetic Alignment of Polymer Macro-Nanodiscs Enables Residual-Dipolar-Coupling-Based High-Resolution Structural Studies by NMR Spectroscopy.
机构信息
Department of Chemistry and Biophysics, Biomedical Engineering, Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI, 48109-1055, USA.
出版信息
Angew Chem Int Ed Engl. 2019 Oct 14;58(42):14925-14928. doi: 10.1002/anie.201907655. Epub 2019 Sep 11.
Abstract
Experimentally measured residual dipolar couplings (RDCs) are highly valuable for atomic-resolution structural and dynamic studies of molecular systems ranging from small molecules to large proteins by solution NMR spectroscopy. Here we demonstrate the first use of magnetic-alignment behavior of lyotropic liquid-crystalline polymer macro-nanodiscs (>20 nm in diameter) as a novel alignment medium for the measurement of RDCs using high-resolution NMR. The easy preparation of macro-nanodiscs, their high stability against pH changes and the presence of divalent metal ions, and their high homogeneity make them an efficient tool to investigate a wide range of molecular systems including natural products, proteins, and RNA.
摘要
通过溶液 NMR 光谱学，实验测量的残余偶极耦合（RDC）对于小至大蛋白质等分子系统的原子分辨率结构和动态研究非常有价值。在这里，我们展示了溶致液晶聚合物大纳米盘（直径>20nm）的磁各向异性行为在使用高分辨率 NMR 测量 RDC 方面的首次应用。大纳米盘易于制备，其对 pH 值变化和二价金属离子的存在具有高稳定性，以及其高均一性，使其成为研究包括天然产物、蛋白质和 RNA 在内的各种分子系统的有效工具。
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