Chemical Biological & Radiological Division, Dstl, Porton Down SP4 0JQ, UK.
Royal Centre for Defence Medicine, Birmingham Research Park, Birmingham B15 2SQ, UK.
Viruses. 2019 Jul 15;11(7):647. doi: 10.3390/v11070647.
Knowledge on haemostatic changes in humans infected with Ebola virus is limited due to safety concerns and access to patient samples. Ethical approval was obtained to collect plasma samples from patients in Sierra Leone infected with Ebola virus over time and samples were analysed for clotting time, fibrinogen, and D-dimer levels. Plasma from healthy volunteers was also collected by two methods to determine effect of centrifugation on test results as blood collected in Sierra Leone was not centrifuged. Collecting plasma without centrifugation only affected D-dimer values. Patients with Ebola virus disease had higher PT and APTT and D-dimer values than healthy humans with plasma collected in the same manner. Fibrinogen levels in patients with Ebola virus disease were normal or lower than values measured in healthy people. Clotting times and D-dimer levels were elevated during infection with Ebola virus but return to normal over time in patients that survived and therefore could be considered prognostic. Informative data can be obtained from plasma collected without centrifugation which could improve patient monitoring in hazardous environments.
由于安全问题和获取患者样本的限制，人们对感染埃博拉病毒的人类的止血变化知之甚少。获得了从塞拉利昂感染埃博拉病毒的患者身上随时间采集血浆样本的伦理批准，并分析了凝血时间、纤维蛋白原和 D-二聚体水平。还通过两种方法从健康志愿者身上采集血浆，以确定离心对测试结果的影响，因为在塞拉利昂采集的血液未离心。不离心采集血浆仅会影响 D-二聚体值。与以相同方式采集血浆的健康人类相比，埃博拉病毒病患者的 PT 和 APTT 以及 D-二聚体值更高。埃博拉病毒病患者的纤维蛋白原水平正常或低于健康人群的测量值。在感染埃博拉病毒期间，凝血时间和 D-二聚体水平升高，但在存活的患者中随着时间的推移恢复正常，因此可以被认为具有预后价值。可以从无需离心的血浆中获得有价值的数据，这可以改善危险环境中患者的监测。
