Lesche Dorothea, Geyer Roland, Lienhard Daniel, Nakas Christos T, Diserens Gaëlle, Vermathen Peter, Leichtle Alexander B
University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland ; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland.
Metabolomics. 2016;12(10):159. doi: 10.1007/s11306-016-1109-3. Epub 2016 Sep 15.
Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.
Determine whether routinely used plasma centrifugation protocols (1500× 10 min; 3000× 5 min) influence non-targeted metabolomic analyses.
Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements.
Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols.
Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.
离心是血浆样本制备不可或缺的步骤，但不同实验室的应用条件可能有所不同。
确定常规使用的血浆离心方案（1500×10分钟；3000×5分钟）是否会影响非靶向代谢组学分析。
采用稀疏偏最小二乘判别分析评估核磁共振波谱（NMR）和高分辨率质谱（HRMS）数据，并与细胞计数测量结果进行比较。
除血小板计数存在显著差异外，我们还发现与不同离心方案相关的NMR和HRMS数据存在实质性变化。
血浆离心过程中即使是微小差异也可能显著影响代谢组学模式，并可能使代谢组学研究产生偏差。
