• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

花生种子发育过程中长链非编码 RNA（lncRNA）的全基因组鉴定和分析。

Genome-wide identification and analysis of long noncoding RNAs (lncRNAs) during seed development in peanut (Arachis hypogaea L.).

机构信息

College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China.

College of Agriculture, Environment and Nutrition Sciences, Tuskegee University, Tuskegee, 36088, AL, USA.

出版信息

BMC Plant Biol. 2020 May 6;20(1):192. doi: 10.1186/s12870-020-02405-4.

DOI:10.1186/s12870-020-02405-4
PMID:32375650
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7203998/
Abstract

BACKGROUND

Long noncoding RNAs (lncRNAs) have several known functions involving various biological regulatory processes in plant. However, the possible roles of lncRNAs during peanut seed development have not been fully explored.

RESULTS

In this study, two peanut recombinant inbred lines (RIL) that differ in seed size were used to investigate comprehensive lncRNA profiles derived from the seed development at 15 and 35 days after flowering (DAF). We identified a total of 9388 known and 4037 novel lncRNAs, from which 1437 were differentially expressed lncRNAs. Interestingly, the expression patterns of a number of lncRNAs can be very different between two closely related inbred lines and these lncRNAs were expressed predominantly in only one RIL at 35 DAF. Some differentially expressed lncRNAs were found related to putative cis-acting target genes and predicted to be involved in transcription, transport, cell division, and plant hormone biosynthesis. The expression patterns of several representative lncRNAs and 12 protein-coding genes were validated by qPCR. Same expression pattern was observed between most lncRNAs and their target genes. 11 lncRNAs, XR_001593099.1, MSTRG.18462.1, MSTRG.34915.1, MSTRG.41848.1, MSTRG.22884.1, MSTRG.12404.1, MSTRG.26719.1, MSTRG.35761.1, MSTRG.20033.1, MSTRG.13500.1, and MSTRG.9304.1 and their cis-acting target genes may play key roles in peanut seed development.

CONCLUSIONS

These results provided new information on lncRNA-mediated regulatory roles in peanut seed development, contributing to the comprehensive understanding of the molecular mechanisms involved in peanut seed development.

摘要

背景

长非编码 RNA（lncRNA）在植物的各种生物调控过程中具有多种已知功能。然而，lncRNA 在花后 15 天和 35 天（DAF）花生种子发育过程中的可能作用尚未得到充分探索。

结果

本研究利用种子大小不同的两个花生重组自交系（RIL），研究了来自种子发育的综合 lncRNA 图谱。共鉴定了 9388 个已知和 4037 个新的 lncRNA，其中 1437 个是差异表达的 lncRNA。有趣的是，许多 lncRNA 的表达模式在两个密切相关的自交系之间可以非常不同，这些 lncRNA 主要在 35 DAF 时仅在一个 RIL 中表达。一些差异表达的 lncRNA 与假定的顺式作用靶基因有关，预测它们参与转录、运输、细胞分裂和植物激素生物合成。通过 qPCR 验证了几个代表性 lncRNA 和 12 个蛋白编码基因的表达模式。大多数 lncRNA 和它们的靶基因之间观察到相同的表达模式。11 个 lncRNA，XR_001593099.1、MSTRG.18462.1、MSTRG.34915.1、MSTRG.41848.1、MSTRG.22884.1、MSTRG.12404.1、MSTRG.26719.1、MSTRG.35761.1、MSTRG.20033.1、MSTRG.13500.1 和 MSTRG.9304.1 及其顺式作用靶基因可能在花生种子发育中发挥关键作用。

结论

这些结果提供了 lncRNA 在花生种子发育中调节作用的新信息，有助于全面了解花生种子发育涉及的分子机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/01856636aef7/12870_2020_2405_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/07dcf223d65d/12870_2020_2405_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/034874734a8c/12870_2020_2405_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/070a40e469c6/12870_2020_2405_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/4710d1a5c6fd/12870_2020_2405_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/6df8de0274b8/12870_2020_2405_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/66b896f61ff6/12870_2020_2405_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/01b082505ba4/12870_2020_2405_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/374384b29f76/12870_2020_2405_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/01856636aef7/12870_2020_2405_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/07dcf223d65d/12870_2020_2405_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/034874734a8c/12870_2020_2405_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/070a40e469c6/12870_2020_2405_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/4710d1a5c6fd/12870_2020_2405_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/6df8de0274b8/12870_2020_2405_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/66b896f61ff6/12870_2020_2405_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/01b082505ba4/12870_2020_2405_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/374384b29f76/12870_2020_2405_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c6c/7203998/01856636aef7/12870_2020_2405_Fig9_HTML.jpg

相似文献

1
Genome-wide identification and analysis of long noncoding RNAs (lncRNAs) during seed development in peanut (Arachis hypogaea L.).花生种子发育过程中长链非编码 RNA（lncRNA）的全基因组鉴定和分析。
BMC Plant Biol. 2020 May 6;20(1):192. doi: 10.1186/s12870-020-02405-4.
2
Discovery, identification, and functional characterization of long noncoding RNAs in Arachis hypogaea L.花生中长链非编码 RNA 的发现、鉴定和功能特征分析
BMC Plant Biol. 2020 Jul 2;20(1):308. doi: 10.1186/s12870-020-02510-4.
3
Genome-Wide Identification and Characterization of Long Non-Coding RNAs in Peanut.花生中长链非编码 RNA 的全基因组鉴定和特征分析。
Genes (Basel). 2019 Jul 15;10(7):536. doi: 10.3390/genes10070536.
4
LncRNA-mediated ceRNA networks provide novel potential biomarkers for peanut drought tolerance.LncRNA 介导的 ceRNA 网络为花生耐旱性提供了新的潜在生物标志物。
Physiol Plant. 2022 Jan;174(1):e13610. doi: 10.1111/ppl.13610. Epub 2021 Dec 9.
5
Genome-wide identification of circular RNAs in peanut (Arachis hypogaea L.).花生（Arachis hypogaea L.）全基因组环状 RNA 的鉴定。
BMC Genomics. 2019 Aug 15;20(1):653. doi: 10.1186/s12864-019-6020-7.
6
Small RNA and Degradome Deep Sequencing Reveals the Roles of microRNAs in Seed Expansion in Peanut ( L.).小RNA和降解组深度测序揭示了microRNA在花生种子膨大过程中的作用。
Front Plant Sci. 2018 Mar 20;9:349. doi: 10.3389/fpls.2018.00349. eCollection 2018.
7
Genome-wide identification and characterization of long non-coding RNAs involved in fruit ripening and the climacteric in Cucumis melo.对参与甜瓜果实成熟和呼吸跃变的长链非编码 RNA 的全基因组鉴定和特征分析。
BMC Plant Biol. 2019 Aug 22;19(1):369. doi: 10.1186/s12870-019-1942-4.
8
Identification and co-expression analysis of long noncoding RNAs and mRNAs involved in the deposition of intramuscular fat in Aohan fine-wool sheep.鉴定和共表达分析与奥汗细毛羊肌内脂肪沉积相关的长非编码 RNA 和 mRNAs。
BMC Genomics. 2021 Feb 1;22(1):98. doi: 10.1186/s12864-021-07385-9.
9
Genome-Wide Identification of lncRNAs During Rice Seed Development.水稻种子发育过程中长链非编码 RNA 的全基因组鉴定
Genes (Basel). 2020 Feb 26;11(3):243. doi: 10.3390/genes11030243.
10
Integrated microRNA and transcriptome profiling reveals a miRNA-mediated regulatory network of embryo abortion under calcium deficiency in peanut (Arachis hypogaea L.).整合 microRNA 和转录组谱分析揭示了缺钙条件下花生（Arachis hypogaea L.）胚胎败育的 miRNA 介导调控网络。
BMC Genomics. 2019 May 21;20(1):392. doi: 10.1186/s12864-019-5770-6.

引用本文的文献

1
LncRNAOmics: A Comprehensive Review of Long Non-Coding RNAs in Plants.长链非编码RNA组学：植物中长链非编码RNA的全面综述
Genes (Basel). 2025 Jun 29;16(7):765. doi: 10.3390/genes16070765.
2
Identification and functional analysis of long non-coding RNA (lncRNA) and metabolites response to mowing in hulless barley (Hordeum vulgare L. var. nudum hook. f.).鉴定和功能分析长非编码 RNA（lncRNA）和代谢物对无壳大麦（Hordeum vulgare L. var. nudum hook. f.）刈割的响应。
BMC Plant Biol. 2024 Jul 12;24(1):666. doi: 10.1186/s12870-024-05334-8.
3
Update on functional analysis of long non-coding RNAs in common crops.

本文引用的文献

1
Transcriptome profiling by RNA-Seq reveals differentially expressed genes related to fruit development and ripening characteristics in strawberries ( × ).通过RNA测序进行转录组分析揭示了与草莓果实发育和成熟特性相关的差异表达基因。
PeerJ. 2018 Jun 27;6:e4976. doi: 10.7717/peerj.4976. eCollection 2018.
2
Identification and expression analysis of cytokinin metabolic genes , and in the biofuel plant .生物燃料植物中细胞分裂素代谢基因、 和 的鉴定与表达分析。
PeerJ. 2018 May 16;6:e4812. doi: 10.7717/peerj.4812. eCollection 2018.
3
Small RNA and Degradome Deep Sequencing Reveals the Roles of microRNAs in Seed Expansion in Peanut ( L.).
常见作物中长链非编码RNA的功能分析进展
Front Plant Sci. 2024 May 30;15:1389154. doi: 10.3389/fpls.2024.1389154. eCollection 2024.
4
Genome-wide identification of long non-coding RNAs and their potential functions in radish response to salt stress.萝卜响应盐胁迫过程中长链非编码RNA的全基因组鉴定及其潜在功能
Front Genet. 2023 Nov 2;14:1232363. doi: 10.3389/fgene.2023.1232363. eCollection 2023.
5
Analysis of lncRNAs in (Tarwi) and Their Potential Role in Drought Response.（塔维）中lncRNAs的分析及其在干旱响应中的潜在作用。
Noncoding RNA. 2023 Aug 23;9(5):48. doi: 10.3390/ncrna9050048.
6
A Review of Classification, Biosynthesis, Biological Activities and Potential Applications of Flavonoids.黄酮类化合物的分类、生物合成、生物活性及潜在应用综述
Molecules. 2023 Jun 25;28(13):4982. doi: 10.3390/molecules28134982.
7
The Classification, Molecular Structure and Biological Biosynthesis of Flavonoids, and Their Roles in Biotic and Abiotic Stresses.类黄酮的分类、分子结构和生物生物合成，以及它们在生物和非生物胁迫中的作用。
Molecules. 2023 Apr 20;28(8):3599. doi: 10.3390/molecules28083599.
8
Genome-Wide Analysis of Long Non-Coding RNAs Related to UV-B Radiation in the Antarctic Moss .南极苔藓中与 UV-B 辐射相关的长非编码 RNA 的全基因组分析
Int J Mol Sci. 2023 Mar 17;24(6):5757. doi: 10.3390/ijms24065757.
9
Roles of non-coding RNAs in the hormonal and nutritional regulation in nodulation and nitrogen fixation.非编码RNA在结瘤和固氮过程中的激素及营养调控作用。
Front Plant Sci. 2022 Oct 18;13:997037. doi: 10.3389/fpls.2022.997037. eCollection 2022.
10
Genome-wide identification and characterization of lncRNAs in sunflower endosperm.向日葵胚乳中长链非编码 RNA 的全基因组鉴定和特征分析。
BMC Plant Biol. 2022 Oct 22;22(1):494. doi: 10.1186/s12870-022-03882-5.
小RNA和降解组深度测序揭示了microRNA在花生种子膨大过程中的作用。
Front Plant Sci. 2018 Mar 20;9:349. doi: 10.3389/fpls.2018.00349. eCollection 2018.
4
Identification of Gossypium hirsutum long non-coding RNAs (lncRNAs) under salt stress.盐胁迫下棉花长非编码 RNA（lncRNA）的鉴定。
BMC Plant Biol. 2018 Jan 25;18(1):23. doi: 10.1186/s12870-018-1238-0.
5
Long noncoding RNAs involve in resistance to Verticillium dahliae, a fungal disease in cotton.长非编码 RNA 参与棉花黄萎病（一种由真菌引起的疾病）的抗性。
Plant Biotechnol J. 2018 Jun;16(6):1172-1185. doi: 10.1111/pbi.12861. Epub 2017 Dec 21.
6
Genome-wide analysis and expression profiling of zinc finger homeodomain (ZHD) family genes reveal likely roles in organ development and stress responses in tomato.锌指同源异型结构域（ZHD）家族基因的全基因组分析和表达谱分析揭示了其在番茄器官发育和应激反应中的可能作用。
BMC Genomics. 2017 Sep 6;18(1):695. doi: 10.1186/s12864-017-4082-y.
7
Variations on a theme in fruit development: the PLE lineage of MADS-box genes in tomato (TAGL1) and other species.果实发育主题的变体：番茄（TAGL1）及其他物种中MADS-box基因的PLE谱系
Planta. 2017 Aug;246(2):313-321. doi: 10.1007/s00425-017-2725-5. Epub 2017 Jun 28.
8
Small RNA profiling and degradome analysis reveal regulation of microRNA in peanut embryogenesis and early pod development.小RNA谱分析和降解组分析揭示了花生胚胎发生和早期荚果发育过程中微小RNA的调控作用。
BMC Genomics. 2017 Mar 2;18(1):220. doi: 10.1186/s12864-017-3587-8.
9
SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 2 controls floral organ development and plant fertility by activating ASYMMETRIC LEAVES 2 in Arabidopsis thaliana.SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 2 通过激活拟南芥中的 ASYMMETRIC LEAVES 2 来控制花器官发育和植物育性。
Plant Mol Biol. 2016 Dec;92(6):661-674. doi: 10.1007/s11103-016-0536-x. Epub 2016 Sep 7.
10
Loss of function at RAE2, a previously unidentified EPFL, is required for awnlessness in cultivated Asian rice.RAE2（一种先前未被鉴定的EPFL）功能丧失是亚洲栽培稻无芒所必需的。
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8969-74. doi: 10.1073/pnas.1604849113. Epub 2016 Jul 27.