A new pyrimidine alkaloid from the roots of (L.) H.Ohashi.

(L.) H.Ohashi, also known as (Fabaceae) is the most important plant in the herbal remedies. The present study focus on the isolation, and studies of the two alkaloids (5-(4-[(methylcarbamoyl) amino]-2-oxopyrimidin-1(2)-yl) tetrahydrofuran-2-yl) methyl methyl carbamate is novel alkaloid and 13-Docosenamide is a known alkaloid. The chemical structures of compounds have been elucidated based on comprehensive techniques like GCMS, IR and NMR. In order to know the molecular mechanisms for the two compounds, molecular docking study has been performed. Both compounds have shown perfect binding affinity to the enzymes TNF α, IL-4, IL-13 and 5 LOX Enzyme. The compounds also exhibited comparable G-scores and Glide energy values in comparison with the standard dexamethasone. In addition both the compounds have been tested for antioxidant assay by using ABTS and DPPH method and the results were compared with standard ascorbic acid.