Mitsuma T, Nogimori T, Hirooka Y
Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Japan.
Exp Clin Endocrinol. 1987 Dec;90(3):365-8. doi: 10.1055/s-0029-1210713.
Effects of growth hormone-releasing hormone (GRH) and corticotropin-releasing hormone (CRH) on the release of immunoreactive thyrotropin-releasing hormone (ir-TRH) from the rat hypothalamus in vitro were studied. The rat hypothalamus was incubated in medium 199 with 1.0 mg/ml of bacitracin (pH 7.4) for 20 min. The amount of ir-TRH release into the medium was measured by radioimmunoassay. The ir-TRH release from the rat hypothalamus was inhibited significantly in a dose-related manner with the addition of GRH or CRH. These findings suggest that GRH and CRH inhibit ir-TRH release from the rat hypothalamus in vitro.
研究了生长激素释放激素（GRH）和促肾上腺皮质激素释放激素（CRH）对体外培养的大鼠下丘脑释放免疫反应性促甲状腺激素释放激素（ir-TRH）的影响。将大鼠下丘脑在含有1.0mg/ml杆菌肽（pH 7.4）的199培养基中孵育20分钟。通过放射免疫测定法测量释放到培养基中的ir-TRH量。添加GRH或CRH后，大鼠下丘脑的ir-TRH释放以剂量相关的方式受到显著抑制。这些发现表明，GRH和CRH在体外抑制大鼠下丘脑的ir-TRH释放。
