生长激素释放激素和促肾上腺皮质激素释放激素对大鼠下丘脑体外释放促甲状腺激素释放激素的影响。

Effects of growth hormone-releasing hormone and corticotropin-releasing hormone on the release of thyrotropin-releasing hormone from the rat hypothalamus in vitro.

作者信息

Mitsuma T, Nogimori T, Hirooka Y

机构信息

Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Japan.

出版信息

Exp Clin Endocrinol. 1987 Dec;90(3):365-8. doi: 10.1055/s-0029-1210713.

DOI:10.1055/s-0029-1210713

PMID:3131150

Abstract

Effects of growth hormone-releasing hormone (GRH) and corticotropin-releasing hormone (CRH) on the release of immunoreactive thyrotropin-releasing hormone (ir-TRH) from the rat hypothalamus in vitro were studied. The rat hypothalamus was incubated in medium 199 with 1.0 mg/ml of bacitracin (pH 7.4) for 20 min. The amount of ir-TRH release into the medium was measured by radioimmunoassay. The ir-TRH release from the rat hypothalamus was inhibited significantly in a dose-related manner with the addition of GRH or CRH. These findings suggest that GRH and CRH inhibit ir-TRH release from the rat hypothalamus in vitro.

摘要

研究了生长激素释放激素（GRH）和促肾上腺皮质激素释放激素（CRH）对体外培养的大鼠下丘脑释放免疫反应性促甲状腺激素释放激素（ir-TRH）的影响。将大鼠下丘脑在含有1.0mg/ml杆菌肽（pH 7.4）的199培养基中孵育20分钟。通过放射免疫测定法测量释放到培养基中的ir-TRH量。添加GRH或CRH后，大鼠下丘脑的ir-TRH释放以剂量相关的方式受到显著抑制。这些发现表明，GRH和CRH在体外抑制大鼠下丘脑的ir-TRH释放。