乙酰胆碱对体外培养的大鼠视网膜促甲状腺激素释放激素释放的影响。

Effects of acetylcholine on the release of thyrotropin-releasing hormone from the rat retina in vitro.

作者信息

Mitsuma T, Hirooka Y, Kayama M, Izeki K, Nogimori T

机构信息

Fourth Department of Internal Medicine, Aichi Medical University, Japan.

出版信息

Exp Clin Endocrinol. 1992;99(2):77-9. doi: 10.1055/s-0029-1211139.

DOI:10.1055/s-0029-1211139

PMID:1639122

Abstract

Effects of acetylcholine on the release of thyrotropin-releasing hormone (TRH) from the rat retina were studied in vitro. The retina was incubated in medium 199 (pH 7.4) with 1.0 mg/ml of bacitracin and 100 micrograms/ml of ascorbic acid (medium) for 20 min. The amount of TRH release into the medium was measured by radioimmunoassay. The TRH release from the rat retina was enhanced significantly in a dose-related manner with the addition of acetylcholine and inhibited with addition of atropine. The stimulatory effect of acetylcholine on TRH release from the retina was blocked with the addition of atropine. The elution profile of methanol-extract of the rat retina was identical to that of synthetic TRH. The findings suggest that the cholinergic system stimulates TRH release from the rat stomach in vitro.

摘要

在体外研究了乙酰胆碱对大鼠视网膜促甲状腺激素释放激素（TRH）释放的影响。将视网膜在含有1.0mg/ml杆菌肽和100μg/ml抗坏血酸的199培养基（pH 7.4）（培养基）中孵育20分钟。通过放射免疫测定法测量释放到培养基中的TRH量。添加乙酰胆碱后，大鼠视网膜的TRH释放以剂量相关的方式显著增强，而添加阿托品则受到抑制。添加阿托品可阻断乙酰胆碱对视网膜TRH释放的刺激作用。大鼠视网膜甲醇提取物的洗脱图谱与合成TRH的洗脱图谱相同。这些发现表明，胆碱能系统在体外刺激大鼠胃中TRH的释放。