合成鳗鱼降钙素在体外刺激大鼠胃释放促甲状腺激素释放激素。
Synthetic eel calcitonin stimulates thyrotropin releasing hormone release from rat stomach in vitro.
作者信息
Mitsuma T, Hirooka Y, Nogimori T
机构信息
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
出版信息
Endocrinol Exp. 1988 Sep;22(3):159-63.
The effect of synthetic eel calcitonin on the release of thyrotropin releasing hormone (TRH) from the rat stomach in vitro was studied. The rat stomach was incubated in medium 199 with 1.0 mg/ml of bacitracin (pH 7.4) for 20 min. The amount of TRH released into the medium was measured by radioimmunoassay. After the addition of synthetic eel calcitonin the release of immunoreactive TRH from the rat stomach in vitro was enhanced significantly in a dose-related manner. Elution profile of acid-methanol-extracted rat stomach on Sephadex G-10 was identical to that of synthetic TRH. The findings suggest that synthetic eel calcitonin stimulated TRH release from the rat stomach in vitro.
研究了合成鳗鱼降钙素对大鼠胃促甲状腺激素释放激素（TRH）体外释放的影响。将大鼠胃在含1.0mg/ml杆菌肽（pH 7.4）的199培养基中孵育20分钟。通过放射免疫测定法测量释放到培养基中的TRH量。加入合成鳗鱼降钙素后，大鼠胃在体外免疫反应性TRH的释放以剂量相关方式显著增强。酸-甲醇提取的大鼠胃在葡聚糖凝胶G-10上的洗脱图谱与合成TRH的洗脱图谱相同。这些发现表明合成鳗鱼降钙素在体外刺激了大鼠胃TRH的释放。
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