多巴胺对体外培养的大鼠视网膜促甲状腺激素释放激素释放的影响。
Effects of dopamine on the release of thyrotropin-releasing hormone from the rat retina in vitro.
作者信息
Mitsuma T, Hirooka Y, Izeki K, Sin K, Nogimori T
机构信息
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
出版信息
Horm Metab Res. 1992 Jun;24(6):263-5. doi: 10.1055/s-2007-1003309.
The effects of dopamine on the release of thyrotropin-releasing hormone (TRH) from the rat retina in vitro were studied. The rat retina was incubated in the medium 199 (pH 7.4) with 1.0 mg/ml of bacitracin and 100 micrograms/ml of ascorbic acid. The amount of TRH release into the medium was measured by radioimmunoassay. The TRH release from the rat retina was inhibited significantly in a dose-related manner with the addition of dopamine, but not with pimozide. The inhibitory effects of dopamine on TRH release from the rat retina were blocked with an addition of pimozide to the medium. The elution profile of methanol-extracted rat retina on sephadex G-10 was identical to that of synthetic TRH. From these findings it is concluded that the dopaminergic system inhibits TRH release from the rat retina in vitro.
研究了多巴胺对体外培养的大鼠视网膜促甲状腺激素释放激素（TRH）释放的影响。将大鼠视网膜置于含有1.0mg/ml杆菌肽和100μg/ml抗坏血酸的199培养基（pH7.4）中孵育。通过放射免疫测定法测量释放到培养基中的TRH量。加入多巴胺后，大鼠视网膜TRH的释放以剂量相关的方式受到显著抑制，但加入匹莫齐特则无此作用。向培养基中加入匹莫齐特可阻断多巴胺对大鼠视网膜TRH释放的抑制作用。甲醇提取的大鼠视网膜在葡聚糖G-10上的洗脱图谱与合成TRH的洗脱图谱相同。从这些发现可以得出结论，多巴胺能系统在体外抑制大鼠视网膜TRH的释放。
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