Department of Obstetrics & Gynecology, General Hospital, Tianjin Medical University, NO.154, Anshan Road, Heping District, Tianjin, 300052, People's Republic of China.
Reproductive Center, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 88 Chang Ling Street, Xi Qing district, Tianjin, 300112, China.
Reprod Biol Endocrinol. 2019 Jul 16;17(1):56. doi: 10.1186/s12958-019-0499-2.
To investigate the therapeutic effects of menstrual blood derived mesenchymal stem cells (MB-MSCs) combined with Bushen Tiaochong recipe (BSTCR) on epirubicin induced premature ovarian failure (POF) in mice.
Twenty-four female C57BL/6 mice of 6-8 weeks were intraperitoneally injected with epirubicin to induce POF, and then they were randomized into 4 groups of 6 mice each and treated with PBS, MB-MSCs, BSTCR, and MB-MSCs combined with BSTCR, respectively. Six mice of the same age were used as controls. Vaginal smear, TUNEL and hematoxylin-eosin staining were to observe estrous cycles, ovarian cell apoptosis and follicles. Enzyme-linked immunosorbent analysis determined serum estradiol, follicle-stimulating hormone (FSH) and anti-Müllerian hormone (AMH) levels. RT-qPCR and Western Blot analysis were to determine GADD45b, CyclinB1, CDC2 and pCDC2 expressions.
Epirubicin treatment resulted in a decrease in the number of primordial, primary, secondary and antral follicles, an increase in the number of atretic follicles and ovarian cell apoptosis, a decrease in estradiol and AMH levels, an increase in FSH levels, and estrous cycle arrest. However, MB-MSCs combined with BSTCR rescued epirubicin induced POF through down-regulating GADD45b and pCDC2 expressions, and up-regulating CyclinB1 and CDC2 expressions. The combined treatment showed better therapeutic efficacy than BSTCR or MB-MSCs alone.
MB-MSCs combined with BSTCR improved the ovarian function of epirubicin induced POF mice, which might be related to the inhibition of GADD45b expression and the promotion of CyclinB1 and CDC2 expressions. The combined treatment had better therapeutic efficacy than BSTCR or MB-MSCs alone.
研究月经血来源间充质干细胞（MB-MSCs）联合补肾调冲方（BSTCR）对阿霉素诱导的小鼠卵巢早衰（POF）的治疗作用。
将 24 只 6-8 周龄 C57BL/6 雌性小鼠腹腔内注射阿霉素诱导 POF，然后随机分为 4 组，每组 6 只，分别给予 PBS、MB-MSCs、BSTCR 和 MB-MSCs 联合 BSTCR 治疗，另取 6 只同月龄小鼠作为对照组。阴道涂片、TUNEL 和苏木精-伊红（HE）染色观察动情周期、卵巢细胞凋亡和卵泡情况。酶联免疫吸附试验检测血清雌二醇（E2）、卵泡刺激素（FSH）和抗苗勒管激素（AMH）水平。实时荧光定量聚合酶链反应（RT-qPCR）和 Western blot 分析检测 GADD45b、CyclinB1、CDC2 和 pCDC2 的表达。
阿霉素处理导致原始卵泡、初级卵泡、次级卵泡和窦卵泡数量减少，闭锁卵泡数量增加，卵巢细胞凋亡增加，E2 和 AMH 水平降低，FSH 水平升高，动情周期停滞。然而，MB-MSCs 联合 BSTCR 通过下调 GADD45b 和 pCDC2 的表达，上调 CyclinB1 和 CDC2 的表达，挽救了阿霉素诱导的 POF。联合治疗的疗效优于 BSTCR 或 MB-MSCs 单独治疗。
MB-MSCs 联合 BSTCR 改善了阿霉素诱导的 POF 小鼠的卵巢功能，这可能与抑制 GADD45b 表达和促进 CyclinB1 和 CDC2 表达有关。联合治疗的疗效优于 BSTCR 或 MB-MSCs 单独治疗。
