Hultberg B M, Daugaard S, Johansen H F, Mouridsen H T, Hou-Jensen K
Department of Pathology, Rigshospitalet, Odense, Denmark.
Histopathology. 1988 Apr;12(4):405-14. doi: 10.1111/j.1365-2559.1988.tb01955.x.
Formalin-fixed, paraffin-embedded material from 15 haemangioendotheliosarcomas and eight malignant haemangiopericytomas was stained with an immunohistochemical technique for the presence of factor VIII-related antigen (F VIII-RAg), actin, laminin and for reactivity with the lectin Ulex europaeus I (UEA-I). Haemangioendotheliosarcomas stained with both F VIII-RAg and UEA-1; however, UEA-I was found to be the more sensitive of the two, reacting also with the poorly differentiated tumours. Haemangiopericytomas reacted negatively with UEA-I; surprisingly, some of these tumours exhibited a weak positivity in staining for F VIII-RAg, possibly supporting the theory about intermediate forms between haemangioendotheliosarcomas and haemangiopericytomas. Laminin was found in most of the haemangioendotheliosarcomas and was useful in illustrating their vascular growth pattern. Haemangiopericytomas also reacted positively for laminin, but the intensity of staining was less pronounced. Positive staining for actin was demonstrated in both tumour types.
采用免疫组织化学技术，对15例血管内皮肉瘤和8例恶性血管外皮细胞瘤的福尔马林固定、石蜡包埋材料进行染色，以检测VIII因子相关抗原（F VIII-RAg）、肌动蛋白、层粘连蛋白的存在以及与荆豆凝集素I（UEA-I）的反应性。血管内皮肉瘤对F VIII-RAg和UEA-1均呈阳性染色；然而，发现UEA-I在两者中更敏感，对低分化肿瘤也有反应。血管外皮细胞瘤对UEA-I呈阴性反应；令人惊讶的是，其中一些肿瘤在F VIII-RAg染色中表现出弱阳性，这可能支持了血管内皮肉瘤和血管外皮细胞瘤之间存在中间形式的理论。在大多数血管内皮肉瘤中发现了层粘连蛋白，它有助于阐明其血管生长模式。血管外皮细胞瘤对层粘连蛋白也呈阳性反应，但染色强度较弱。两种肿瘤类型对肌动蛋白均呈阳性染色。
