Laboratory of Medicinal and Aromatics Plant, Biotechnology Centre of Borj-Cedria, Hammam-Lif, Tunisia.
General and Organic Chemistry-Volatolomics, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
Phytochem Anal. 2020 Jan;31(1):37-45. doi: 10.1002/pca.2864. Epub 2019 Jul 16.
The consumption of health-promoting products such as oil seeds may improve human health and prevent certain diseases. Carvi seeds have the potential to produce oil with nutritional and functional properties rich in active compounds.
To extract bioactive lipids from Carum carvi seeds using green methodologies.
Supercritical-carbon dioxide (Sc-CO ) and ethanol as co-solvent and bio-based solvent 2-methyltetrahydrofuran (MeTHF) were used to extract the oil from Carum carvi. The yield, the chemical composition, as well as antioxidant and anti-inflammatory activities of green extracted oils were investigated and compared to those obtained with conventional methods (hexane and Folch system).
MeTHF extraction gave higher oil yield than that obtained by hexane. Fatty acids composition of the two obtained green extracted oils was similar to conventional extracted ones where petroselinic (39-43%), linoleic (29-31%) and oleic (19-21%) acids were the major compounds. Furthermore, MeTHF and Sc-CO green extracted oils were enriched of bioactive compounds including sterols (5.4 and 7.3 mg/g oil) and total polyphenols (9.3 and 7.6 mg GAE/g oil) which were correlated to enhanced antiradical capacity. Moreover, the green extracted oils exhibited high anti-inflammatory capacity inhibiting nitric oxide (NO) release in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages with IC values of 28 and 24 μg/mL.
Green solvents are a good alternative to petroleum solvents to recover oil from carvi seeds with high amount of nutritionally important fatty acids, along with significant antioxidant and anti-inflammatory potential.
食用具有促进健康作用的产品，如油籽，可能有益于人体健康，并预防某些疾病。 芫荽籽具有生产具有营养和功能性的油的潜力，其中富含活性化合物。
使用绿色方法从芫荽籽中提取生物活性脂质。
超临界二氧化碳（Sc-CO ）和乙醇作为共溶剂和生物基溶剂 2-甲基四氢呋喃（MeTHF）被用于从芫荽籽中提取油。 考察并比较了绿色提取油的产率、化学成分以及抗氧化和抗炎活性与常规方法（己烷和Folch 系统）获得的结果。
MeTHF 提取的产率高于己烷。 两种绿色提取油的脂肪酸组成与常规提取油相似，其中芹菜烯酸（39-43％）、亚油酸（29-31％）和油酸（19-21％）是主要化合物。 此外，MeTHF 和 Sc-CO 绿色提取油富含生物活性化合物，包括甾醇（5.4 和 7.3 mg/g 油）和总多酚（9.3 和 7.6 mg GAE/g 油），这与增强的自由基清除能力有关。 此外，绿色提取油表现出高的抗炎能力，抑制脂多糖（LPS）诱导的 RAW 264.7 巨噬细胞中一氧化氮（NO）的释放，IC 值为 28 和 24 μg/mL。
绿色溶剂是从芫荽籽中提取油的石油溶剂的良好替代品，可获得具有重要营养价值的脂肪酸含量高的油，同时具有显著的抗氧化和抗炎潜力。
