Bourgou Soumaya, Bettaieb Rebey Iness, Ben Kaab Sofiene, Hammami Majdi, Dakhlaoui Sarra, Sawsen Selmi, Msaada Kamel, Isoda Hiroko, Ksouri Riadh, Fauconnier Marie-Laure
Laboratory of Medicinal and Aromatics Plant, Biotechnology Center of Borj-Cedria, BP 901, 2050 Hammam-Lif, Tunisia.
Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030 Gembloux, Belgium.
Foods. 2021 Jun 28;10(7):1493. doi: 10.3390/foods10071493.
A comparative study of bioactive lipids extraction from black cumin ( L.) and basil ( L.) seeds using conventional petroleum-based solvent and green solvent 2-methyltetrahydrofuran (MeTHF) was performed. MeTHF extraction allowed obtaining the highest oil yield in black cumin (34%). Regarding fatty acids composition, linoleic acid (61%) and α-linolenic (78%) were relevant in black cumin and basil green and conventionally extracted oils, respectively. Besides, MeTHF allowed obtaining higher tocopherols and total phenolics contents in black cumin (400 mg/kg of oil and 12 mg EGA/g oil) and basil (317 mg/kg oil and 5 mg EGA/g oil) compared to hexane-extracted ones. The content of major phenolic compounds in the two seed oils, -hydroxycinnamic acid, rosmarinic acid, and thymol was enhanced by MeTHF extraction. Furthermore, MeTHF-extracted oils possess stronger antioxidant activities (radical scavenging, total antioxidant, and β-carotene bleaching activities) and high and similar anti-inflammatory capacity to hexane-extracted oils. In conclusion, the results revealed that MeTHF is efficient to replace hazardous solvents to extract oil from black cumin and basil seeds rich in compounds relevant to the human diet, including essential polyunsaturated fatty acids (n-6 and n-3), tocopherols, and phenolic compounds with improved biological activities.
进行了一项比较研究，使用传统的石油基溶剂和绿色溶剂2-甲基四氢呋喃（MeTHF）从黑种草（L.）和罗勒（L.）种子中提取生物活性脂质。MeTHF提取法能使黑种草的出油率最高（34%）。关于脂肪酸组成，亚油酸（61%）和α-亚麻酸（78%）分别在黑种草以及罗勒的绿色提取油和传统提取油中占比较大。此外，与己烷提取的油相比，MeTHF提取法能使黑种草（400 mg/kg油和12 mg EGA/g油）和罗勒（317 mg/kg油和5 mg EGA/g油）中的生育酚和总酚含量更高。MeTHF提取法提高了两种种子油中主要酚类化合物——对羟基肉桂酸、迷迭香酸和百里香酚的含量。此外，MeTHF提取的油具有更强的抗氧化活性（自由基清除、总抗氧化和β-胡萝卜素漂白活性），并且与己烷提取的油具有相似的高抗炎能力。总之，结果表明MeTHF能有效替代有害溶剂，从富含对人类饮食有益化合物的黑种草和罗勒种子中提取油脂，这些化合物包括必需多不饱和脂肪酸（n-6和n-3）、生育酚以及具有改善生物活性的酚类化合物。
