七叶灵诱导人结肠癌 LoVo 细胞周期阻滞和凋亡。
Esculetin induces cell cycle arrest and apoptosis in human colon cancer LoVo cells.
机构信息
Department of Biological Sciences, Gachon University, Seongnam-si, Gyeonggi-do, Republic of Korea.
Department of Bio and Chemical Engineering, Hongik University, Sejong, Republic of Korea.
出版信息
Environ Toxicol. 2019 Oct;34(10):1129-1136. doi: 10.1002/tox.22815. Epub 2019 Jul 16.
We investigated the anti-cancer effects of ESC in human colon cancer LoVo cells. Cell counting assay results showed that ESC inhibited the proliferation of LoVo cells. Cell cycle arrest results showed that cell cycle was arrested during the G0/G1 phase in the ESC-treated LoVo cells. Western blot results showed that the cell cycle inhibitory proteins p53, p27, and p21 were increased, and cyclin D1, the cell cycle progressive protein, was decreased. Sp1 is a transcription factor regulating cell proliferation, was decreased in the ESC-treated LoVo cells. Annexin V/propidium iodide staining results showed that ESC induces apoptosis in LoVo cells. Western blot results showed that Bax, cleaved caspase -3, -7, -9, and poly(ADP-ribose) polymerase, which are proapoptotic proteins, were increased and the antiapoptotic protein Bcl-2 was decreased. Taken together, ESC induced apoptosis and has an anti-cancer effect in LoVo cells.
我们研究了 ESC 在人结肠癌细胞 LoVo 中的抗癌作用。细胞计数检测结果表明，ESC 抑制 LoVo 细胞的增殖。细胞周期阻滞结果表明，ESC 处理的 LoVo 细胞的细胞周期被阻滞在 G0/G1 期。Western blot 结果表明，细胞周期抑制蛋白 p53、p27 和 p21 增加，细胞周期推进蛋白 cyclin D1 减少。Sp1 是调节细胞增殖的转录因子，在 ESC 处理的 LoVo 细胞中减少。Annexin V/碘化丙啶染色结果表明，ESC 诱导 LoVo 细胞凋亡。Western blot 结果表明，促凋亡蛋白 Bax、cleaved caspase-3、-7、-9 和多聚（ADP-核糖）聚合酶增加，抗凋亡蛋白 Bcl-2 减少。综上所述，ESC 诱导 LoVo 细胞凋亡并具有抗癌作用。
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