Department of Biomedicine - Unit of Biochemistry, Faculty of Medicine of Porto, University of Porto, Porto, Portugal.
Food Funct. 2019 Aug 1;10(8):4566-4576. doi: 10.1039/c9fo01142k. Epub 2019 Jul 17.
Intake of fructose-containing sugars is epidemiological and experimentally linked to metabolic syndrome (MS). We recently verified that the dietary polyphenol chrysin was able to abolish some of the metabolic changes induced by fructose-feeding in the rat. Because the role of the intestine upon fructose-induced MS is poorly understood, we decided to investigate the influence of fructose, in vivo, on the intestinal environment and the ability of chrysin to interfere with the putative observed changes. For this, adult male Sprague-Dawley rats were treated for 18 weeks as follows: (A) tap water (CONT), (B) tap water and chrysin (100 mg kg day) (CHRY), (C) 10% fructose in tap water (FRUCT), and (D) 10% fructose in tap water and chrysin (100 mg kg day) (FRUCT + CHRY). Our findings show that the relative expression of SGLT1 and GLUT2 mRNA were not affected by fructose-feeding and/or chrysin. In contrast, GLUT5 mRNA expression was markedly increased in fructose-fed animals, and this effect was reduced by chrysin. However, the apparent permeability to C-FRUCT was markedly and similarly decreased in FRUCT, CHRY and FRUCT + CHRY rats. Jejunal villus width and crypt depth were significantly higher in FRUCT and FRUCT + CHRYS rats, respectively. Finally, chrysin did not alter gut microbiota composition, but fructose significantly increased Lactobacillus and E. coli. Moreover, FRUCT + CHRY rats had an increase on the Firmicutes to Bacteroidetes ratio. This is the first report showing that chrysin is able to interfere with the effects of fructose at the intestinal level, which may contribute to the fructose-induced MS features.
果糖的摄入与代谢综合征（MS）在流行病学和实验上有关。我们最近证实，膳食多酚白杨素能够消除果糖喂养大鼠的一些代谢变化。由于肠道在果糖引起的 MS 中的作用尚未得到充分了解，我们决定研究果糖在体内对肠道环境的影响，以及白杨素干扰观察到的变化的能力。为此，成年雄性 Sprague-Dawley 大鼠接受以下 18 周的治疗：（A）自来水（CONT），（B）自来水和白杨素（100mgkg 天）（CHRY），（C）10%果糖在自来水中（FRUCT），和（D）10%果糖在自来水中和白杨素（100mgkg 天）（FRUCT+CHRY）。我们的研究结果表明，SGLT1 和 GLUT2 mRNA 的相对表达不受果糖喂养和/或白杨素的影响。相比之下，GLUT5 mRNA 的表达在果糖喂养的动物中明显增加，而白杨素则降低了这种作用。然而，C-FRUCT 的表观通透性在 FRUCT、CHRY 和 FRUCT+CHRY 大鼠中明显且相似地降低。空肠绒毛宽度和隐窝深度在 FRUCT 和 FRUCT+CHRYS 大鼠中分别显著升高。最后，白杨素并没有改变肠道微生物群的组成，但果糖显著增加了乳杆菌和大肠杆菌。此外，FRUCT+CHRY 大鼠的厚壁菌门与拟杆菌门的比例增加。这是第一个报道显示白杨素能够在肠道水平上干扰果糖的作用，这可能有助于果糖诱导的 MS 特征。
