Rubin R H, Young L S, Hansen W P, Nedelman M, Wilkinson R, Nelles M J, Callahan R, Khaw B A, Strauss H W
Department of Radiology, Massachusetts General Hospital, Boston 02114.
J Nucl Med. 1988 May;29(5):651-6.
To determine if radiolabeled specific antibodies directed against bacterial antigens could be used to detect sites of infection, gamma camera imaging studies were performed in animals infected with Pseudomonas aeruginosa. Murine monoclonal antibodies (Mabs) directed against Fisher Immunotype 1 Pseudomonas aeruginosa and a nonmicrobial, nonmammalian haptene, p-arsanilic acid, were labeled with 125I by the lodogen-Bead method. Unilateral, deep thigh infections were created by innoculation with 2 X 10(8) Fisher Immunotype 1 P. aeruginosa. Twenty-four hours later, one of the radiolabeled antibodies was injected intravenously at a dose of 0.25 mg/kg (100-150 microCi). Serial gamma imaging was then carried out beginning at 4 hr and at approximately 24-hr intervals thereafter. Beginning as early as 4 hr postinjection, the area of inflammation could be visualized with either the specific or nonspecific Mab, with the images continuing to intensify until 24-48 hr postinjection. At 48 hr, the contrast between lesion and background with the nonspecific Mab began to fade, while the contrast in the specific Mab-generated images continued to intensify until approximately 192 hr postinjection. Clear-cut differentiation between specific and nonspecific Mab-generated images was possible by 72 hr postinjection. We conclude that specific immune imaging of localized infection with Mab's directed against specific microbial antigens is possible and should be clinically useful. In addition, images created by the localization of immunoglobulin non-specifically at the site of inflammation in the first 24-48 hr postinjection may also provide useful information as to the anatomic location of hidden abscesses.
为了确定针对细菌抗原的放射性标记特异性抗体是否可用于检测感染部位，我们对感染铜绿假单胞菌的动物进行了γ相机成像研究。通过碘珠法用¹²⁵I标记针对费希尔免疫型1铜绿假单胞菌的鼠单克隆抗体（Mab）和一种非微生物、非哺乳动物半抗原对氨基苯磺酸。通过接种2×10⁸费希尔免疫型1铜绿假单胞菌造成单侧大腿深部感染。24小时后，以0.25mg/kg（100 - 150微居里）的剂量静脉注射其中一种放射性标记抗体。然后从4小时开始并在此后约24小时间隔进行连续γ成像。早在注射后4小时，用特异性或非特异性Mab均可观察到炎症区域，图像持续增强直至注射后24 - 48小时。在48小时时，非特异性Mab产生的病变与背景之间的对比度开始减弱，而特异性Mab产生的图像中的对比度持续增强直至注射后约192小时。注射后72小时可实现特异性和非特异性Mab产生的图像之间的清晰区分。我们得出结论，用针对特定微生物抗原的Mab对局部感染进行特异性免疫成像具有可行性，且在临床上应具有实用性。此外，注射后最初24 - 48小时免疫球蛋白在炎症部位非特异性定位所产生的图像也可能提供有关隐匿性脓肿解剖位置的有用信息。
