Stavropol Research Anti-Plague Institute, 13-15 Sovetskaya Str, Stavropol, 355035, Russia.
Stavropol State Agrarian University, Stavropol, 355017, Russian Federation.
BMC Microbiol. 2019 Jul 17;19(1):165. doi: 10.1186/s12866-019-1542-3.
Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B. anthracis from the territory of Russia. In this study, we report the use of whole genome sequencing (WGS) and bioinformatics analysis to characterize B. anthracis 81/1 strain isolated in Russia in 1969 from a person during an outbreak of the disease in the Stavropol region.
We used 232 B. anthracis genomes, which are currently available in the GenBank database, to determine the place of the Russian isolate in the global phylogeny of B. anthracis. The studied strain was characterized by PCR-based genetic methods, such as Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA), canonical single nucleotide polymorphisms (canSNP), as well as the method of full-genomic analysis of nucleotide polymorphisms (wgSNP). The results indicate that the Russian B. anthracis 81/1 strain belongs to Trans-Eurasion (TEA) group, the most representative in the world.
In this study, the full genomic sequence of virulent B. anthracis strain from Russia was characterized for the first time. As a result of complex phylogenetic analysis, the place of this isolate was determined in the global phylogenetic structure of the B. anthracis population, expanding our knowledge of anthrax phylogeography in Russia.
炭疽是一种由革兰氏阳性细菌炭疽芽孢杆菌引起的人畜共患病。在俄罗斯，有超过 3.5 万个炭疽固定不利地点。与此同时，关于俄罗斯境内炭疽杆菌分离株的出版物却很少。在这项研究中，我们报告了使用全基因组测序（WGS）和生物信息学分析来表征 1969 年在俄罗斯斯塔夫罗波尔地区炭疽病爆发期间从一名患者中分离出的炭疽芽孢杆菌 81/1 株。
我们使用了目前可在 GenBank 数据库中获得的 232 个炭疽芽孢杆菌基因组，以确定俄罗斯分离株在炭疽芽孢杆菌全球系统发育中的位置。该研究菌株通过基于 PCR 的遗传方法进行了特征描述，例如多位点可变数串联重复分析（MLVA）、典型单核苷酸多态性（canSNP）以及全基因组核苷酸多态性分析（wgSNP）方法。结果表明，俄罗斯炭疽芽孢杆菌 81/1 株属于 Trans-Eurasion（TEA）组，这是世界上最具代表性的一组。
在这项研究中，首次对来自俄罗斯的毒力炭疽芽孢杆菌菌株的全基因组序列进行了特征描述。通过复杂的系统发育分析，确定了该分离株在炭疽芽孢杆菌种群全球系统发育结构中的位置，扩展了我们对俄罗斯炭疽病 phylogeography 的认识。
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