Stavropol Research Anti-Plague Institute, 13-15 Sovetskaya Street, 355035, Stavropol, Russia.
Irkutsk Research Anti-Plague Institute, Irkutsk, Russian Federation, 664047.
BMC Genomics. 2018 May 10;19(1):353. doi: 10.1186/s12864-018-4762-2.
Brucellosis is a bacterial zoonotic disease. Annually in the world more than 500,000 new cases of brucellosis in humans are registered. In this study, we propose an evolutionary model of the historical distribution of B. melitensis based on the full-genomic SNP analysis of 98 strains.
We performed an analysis of the SNP of the complete genomes of 98 B. melitensis strains isolated in different geographical regions of the world to obtain relevant information on the population structure, genetic diversity and the evolution history of the species. Using genomic sequences of 21 strains of B. melitensis isolated in Russia and WGS data from the NCBI database, it was possible to identify five main genotypes and 13 species genotypes for analysis. Data analysis based on the Bayesian Phylogenetics and Phylogeography method allowed to determine the regions of geographical origin and the expected pathways of distribution of the main lines (genotypes and subgenotypes) of the pathogen.
Within the framework of our study, the model of global evolution and phylogeography of B. melitensis strains isolated in various regions of the planet was proposed for the first time. The sets of unique specific SNPs described in our study, for all identified genotypes and subgenotypes, can be used to develop new bacterial typing and identification systems for B. melitensis.
布鲁氏菌病是一种细菌性人畜共患病。全球每年有超过 50 万人新感染布鲁氏菌病。在这项研究中，我们基于对 98 株细菌的全基因组 SNP 分析，提出了 B. melitensis 历史分布的进化模型。
我们对从世界不同地理区域分离出的 98 株 B. melitensis 进行了全基因组 SNP 分析，以获取有关该物种的种群结构、遗传多样性和进化历史的相关信息。使用俄罗斯分离的 21 株 B. melitensis 的基因组序列和来自 NCBI 数据库的 WGS 数据，我们可以鉴定出五个主要基因型和 13 个种基因型进行分析。基于贝叶斯系统发生和系统地理学方法的数据分析，确定了主要谱系（基因型和亚型）的地理起源区域和预期分布途径。
在我们的研究框架内，首次提出了在地球上不同地区分离的 B. melitensis 菌株的全球进化和系统地理学模型。我们描述的所有鉴定基因型和亚型的独特特定 SNP 集，可用于开发 B. melitensis 的新细菌分型和鉴定系统。
