Schmid K M, Patterson G W
Department of Botany, University of Maryland, College Park 20742.
Lipids. 1988 Mar;23(3):248-52. doi: 10.1007/BF02535466.
Cyclopropenoid fatty acids (CPE) isolated from Sterculia foetida oil by urea clathration and reverse phase high performance liquid chromatography (HPLC) were introduced into fungal cultures. Stearate levels in phospholipids and triacylglycerols from Ustilago maydis sporidia rose considerably in response to 30 microM CPE. In addition, CPE themselves were incorporated into glycerolipid fractions. Sterol composition was unaffected. Changes in lipid composition were accompanied by inhibition of dry weight accumulation and sporidial number. Treated sporidia showed irregular wall deposition and a branched morphology. Oleate alleviated CPE effects on growth and morphology. Hyphal extension by Rhizoctonia solani was inhibited somewhat by 30 microM sterculate, while Fusarium oxysporum showed no appreciable response. Although CPE appeared to inhibit fatty acid desaturation by F. oxysporum, gross increases in the proportion of stearate were limited to the triacylglycerol fraction during 30 microM treatments. The possibility that the CPE synthesized by plants serve as antifungal agents is discussed.
通过尿素包合法和反相高效液相色谱法（HPLC）从苹婆油中分离得到的环丙烯脂肪酸（CPE）被引入到真菌培养物中。玉米黑粉菌孢子中的磷脂和三酰甘油中的硬脂酸水平因30微摩尔CPE而显著升高。此外，CPE自身也被整合到甘油脂部分。甾醇组成未受影响。脂质组成的变化伴随着干重积累和孢子数量的抑制。经处理的孢子显示出不规则的壁沉积和分支形态。油酸减轻了CPE对生长和形态的影响。30微摩尔硬脂酸对立枯丝核菌的菌丝延伸有一定抑制作用，而尖孢镰刀菌则无明显反应。尽管CPE似乎抑制了尖孢镰刀菌的脂肪酸去饱和作用，但在30微摩尔处理期间，硬脂酸比例的总体增加仅限于三酰甘油部分。文中讨论了植物合成的CPE作为抗真菌剂的可能性。
