Mazuran R, Rabatić S, Sabioncello A, Dekaris D
Allergy. 1979 Feb;34(1):25-34. doi: 10.1111/j.1398-9995.1979.tb01997.x.
Immunological functions of the pharyngeal tonsil, palatine tonsils and blood leucocytes of children undergoing tonsillectomy were evaluated by determining T or B lymphocytes, the response to mitogens, and the cell-mediated immunological responses to tuberculin. In all the test systems used similar results were obtained with cells derived from either the palatine or pharyngeal tonsils. The mean percentage of T lymphocytes was significantly higher in the peripheral blood than in tonsils, but the reverse was true of B lymphocytes. The reaction to PHA was lower in tonsillar cell culture than in blood cell culture, but tonsillar cells reacted better to Con A than blood cells. In lymphocyte transformation tests tonsillar cells reacted to specific antigen (tuberculin) and this reaction was significantly higher than that of the parallelly tested blood lymphocytes. Further, in about 50% of the children tested, tuberculin caused migration inhibition of the mixture containing tonsillar cells and guinea pig peritoneal cells. Surprisingly, nearly identical results were obtained if migration inhibition test was performed with tonsillar cells alone. Consequently, poorly migrating tonsillar cells are nevertheless usable for direct migration inhibition testing.
通过测定T或B淋巴细胞、对有丝分裂原的反应以及对结核菌素的细胞介导免疫反应，评估了接受扁桃体切除术的儿童的咽扁桃体、腭扁桃体和血液白细胞的免疫功能。在所有使用的测试系统中，来自腭扁桃体或咽扁桃体的细胞都获得了相似的结果。外周血中T淋巴细胞的平均百分比显著高于扁桃体，但B淋巴细胞的情况则相反。扁桃体细胞培养物对PHA的反应低于血细胞培养物，但扁桃体细胞对Con A的反应优于血细胞。在淋巴细胞转化试验中，扁桃体细胞对特定抗原（结核菌素）有反应，且该反应显著高于平行测试的血液淋巴细胞。此外，在约50%接受测试的儿童中，结核菌素导致含有扁桃体细胞和豚鼠腹腔细胞的混合物出现迁移抑制。令人惊讶的是，如果仅用扁桃体细胞进行迁移抑制试验，也能获得几乎相同的结果。因此，迁移能力较差的扁桃体细胞仍可用于直接迁移抑制试验。
