Minimizing off-target effects in CRISPR-Cas9 genome editing.
作者信息
Chen Shi-Jie
机构信息
Department of Physics, Department of Biochemistry, and UM Informatics Institute, University of Missouri, Columbia, MO, 65211, USA.
出版信息
Cell Biol Toxicol. 2019 Oct;35(5):399-401. doi: 10.1007/s10565-019-09486-4. Epub 2019 Jul 17.
Abstract
摘要
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本文引用的文献
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Off-target genome editing: A new discipline of gene science and a new class of medicine.脱靶基因组编辑：基因科学的新学科和一类新药。
Cell Biol Toxicol. 2019 Jun;35(3):179-183. doi: 10.1007/s10565-019-09475-7. Epub 2019 Apr 27.
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Increasing the specificity of CRISPR systems with engineered RNA secondary structures.利用工程化 RNA 二级结构提高 CRISPR 系统的特异性。
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Unified energetics analysis unravels SpCas9 cleavage activity for optimal gRNA design.统一的能量分析揭示 SpCas9 切割活性，以优化 gRNA 设计。
Proc Natl Acad Sci U S A. 2019 Apr 30;116(18):8693-8698. doi: 10.1073/pnas.1820523116. Epub 2019 Apr 15.
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Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.增强型校对控制CRISPR-Cas9靶向准确性。
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CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment.CRISPR-Cas9 切割效率与靶 sgRNA 折叠稳定性密切相关：从物理机制到脱靶评估。
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High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.具有不可检测的全基因组脱靶效应的高保真CRISPR-Cas9核酸酶。
Nature. 2016 Jan 28;529(7587):490-5. doi: 10.1038/nature16526. Epub 2016 Jan 6.
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Rationally engineered Cas9 nucleases with improved specificity.具有更高特异性的理性设计的Cas9核酸酶。
Science. 2016 Jan 1;351(6268):84-8. doi: 10.1126/science.aad5227. Epub 2015 Dec 1.
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Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.二聚体 CRISPR RNA 引导的 FokI 核酸酶可实现高度特异性的基因组编辑。
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Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification.将无催化活性的 Cas9 融合到 FokI 核酸内切酶可提高基因组修饰的特异性。
Nat Biotechnol. 2014 Jun;32(6):577-582. doi: 10.1038/nbt.2909. Epub 2014 Apr 25.
10
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.利用 CRISPR-Cas9 切口酶实现高效基因组修饰，最小化脱靶效应。
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