Sección Virus Oncogénicos, Subdepto. de Enfermedades Virales, Instituto de Salud Pública de Chile, Ñuñoa, Santiago, Chile.
Dirección de Atención Primaria Santiago, Servicio de Salud Metropolitano Central, Santiago, Chile.
J Med Virol. 2019 Nov;91(11):2009-2015. doi: 10.1002/jmv.25551. Epub 2019 Jul 25.
Human papillomavirus (HPV) is the etiological agent of cervical cancer. Also, HPV has been associated with anogenital cancer, oropharyngeal cancer, genital warts, and other dermatological diseases. HPV infects epithelial cells and their replication is closely linked to epithelial differentiation. The presence of HPV DNA in peripheral blood mononuclear cells (PBMC) has been reported in some patients with head and neck cancer, cervical cancer, and other genital diseases. However, the presence of HPV DNA in blood in asymptomatic subjects is still unresolved. The objective of this study was to evaluate the presence of HPV DNA in PBMC from asymptomatic blood donors. Blood samples were collected from 207 healthy Chilean blood donors. Genomic DNA was extracted from PBMC and HPV DNA detection was performed by real-time quantitative polymerase chain reaction assays with GP5+/6+ primers. HPV typing was carried out by genetic sequencing of a 140 to 150 bp fragment of the L1 gene. HPV DNA was detected in 6.8% (14/207) of blood donors. Single HPV infections were detected in seven blood donors. High-risk HPV was found in 6.3% (13/207) of cases: nine blood donors were infected with HPV-16, five with HPV-18, two with HPV-51, and one case was infected with either 32, 33, 45, 59, 66, 70, or 82. The median viral load value was 21.3 copies/mL blood or 13.4 HPV (+) cells per 10 PBMC. These results show that HPV DNA is present in PBMC from healthy blood donors and it suggests that blood could be a new route of HPV dissemination.
人乳头瘤病毒（HPV）是宫颈癌的病因。此外，HPV 还与肛门生殖器癌、口咽癌、生殖器疣和其他皮肤病有关。HPV 感染上皮细胞，其复制与上皮分化密切相关。一些头颈部癌症、宫颈癌和其他生殖器疾病患者的外周血单个核细胞（PBMC）中存在 HPV DNA 已被报道。然而，无症状受试者血液中 HPV DNA 的存在仍未得到解决。本研究旨在评估无症状献血者 PBMC 中 HPV DNA 的存在。从 207 名智利健康献血者中采集血样。从 PBMC 中提取基因组 DNA，并通过 GP5+/6+引物的实时定量聚合酶链反应（PCR）检测 HPV DNA。通过 L1 基因的 140-150bp 片段的遗传测序进行 HPV 分型。在 6.8%（14/207）的献血者血液中检测到 HPV DNA。在 7 名献血者中检测到单一 HPV 感染。在 6.3%（13/207）的病例中发现高危 HPV：9 名献血者感染 HPV-16，5 名感染 HPV-18，2 名感染 HPV-51，1 名感染 HPV-32、33、45、59、66、70 或 82。病毒载量中位数为 21.3 拷贝/ml 血液或每 10 PBMC 中有 13.4 个 HPV（+）细胞。这些结果表明 HPV DNA 存在于健康献血者的 PBMC 中，这表明血液可能是 HPV 传播的新途径。
