Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, Tainan, 701, Taiwan.
Department of Chemical Engineering and Chemistry, Mapúa Institute of Technology, 658 Muralla St., Intramuros, Manila, 1002, Philippines.
Chemosphere. 2019 Dec;236:124291. doi: 10.1016/j.chemosphere.2019.07.022. Epub 2019 Jul 8.
This study evaluated biological treatment of dimethyl sulfoxide (DMSO)-containing wastewater from semiconductor industry under aerobic and anaerobic conditions. DMSO concentration as higher as 1.5 g/L did not inhibit DMSO degradation efficiency in aerobic membrane bioreactor (MBR), while specific DMSO degradation rate at different initial DMSO-to-biomass (S/X) ratios from batch tests seemed to follow the Haldane-type kinetics. According to the microbial community analysis, Proteobacteria decreased from 88.2% to 26% as influent DMSO concentration increased, while Bacteroidetes, Parcubacteria, Saccharibacteria increased. Within the Bacteroidetes class, Flavobacterium and Laribacter genus significantly increased from less than 0.05%-26.8% and 13.4%, respectively, which might both be related to the DMS degradation. Hyphomicrobium and Thiobacillus, known as aerobic DMSO and DMS degraders, instead, decreased at higher DMSO conditions. Under methanogenic conditions, batch results implied DMSO concentrations higher than 3 g/L could be inhibitory, while DMSO and COD removal achieved 100% and 93%, respectively, using a pilot-scale anaerobic fluidized bed membrane bioreactor (AFMBR) with influent DMSO below 1.5 g/L. Results of terminal restriction fragment length polymorphism (TRFLP) analysis targeting on mcrA functional gene revealed that Methanomethylovorans sp. was dominant in AFMBR after 54 days of operation, indicating its importance on degrading DMS and mathanethiol (MT).
本研究评估了在有氧和厌氧条件下对半导体工业含二甲基亚砜（DMSO）废水进行生物处理的效果。DMSO 浓度高达 1.5g/L 时，并未抑制有氧膜生物反应器（MBR）中 DMSO 的降解效率，而批处理试验中不同初始 DMSO-生物质（S/X）比下的特定 DMSO 降解速率似乎遵循 Haldane 型动力学。根据微生物群落分析，随着进水 DMSO 浓度的增加，变形菌门从 88.2%减少到 26%，而拟杆菌门、Parcubacteria 门和 Saccharibacteria 门增加。在拟杆菌门中，黄杆菌属和拉雷菌属的丰度分别从低于 0.05%-26.8%和 13.4%显著增加，这可能都与 DMS 的降解有关。好氧 DMSO 和 DMS 降解菌（如 Hyphomicrobium 和 Thiobacillus）反而在较高 DMSO 条件下减少。在产甲烷条件下，批处理结果表明，当 DMSO 浓度高于 3g/L 时可能具有抑制作用，而当进水 DMSO 低于 1.5g/L 时，采用中试规模的厌氧流化床膜生物反应器（AFMBR），可实现 100%的 DMSO 和 93%的 COD 去除率。针对 mcrA 功能基因的末端限制性片段长度多态性（TRFLP）分析结果表明，在 54 天的运行后，Methanomethylovorans sp. 在 AFMBR 中占主导地位，表明其在降解 DMS 和甲硫醇（MT）方面的重要性。
