Department of Public Health and Paediatrics, University of Torino, Piazza Polonia 94, 10126 Torino, Italy.
Int J Environ Res Public Health. 2019 Jul 17;16(14):2546. doi: 10.3390/ijerph16142546.
Bioaerosol exposure linked to the bioenergy production from waste and its effects on human health in occupational and residential environments has rising interest nowadays. The health risk associated with the exposure includes mainly infective diseases, allergies, chronic bronchitis, and obstructive pulmonary disease. A risk assessment's critical point is the bioaerosol quality and quantity characterization. The aim of this study is to evaluate the application of different methods for the analysis of bioaerosol sampled in bioenergy plants. This study involved six Italian plants for the treatment of biomasses and energy production. Bioaerosol cultural evaluation was performed, by Surface Air System (SAS) sampler, and DNA was extracted from PM0.49 samples and Low Melting Agar plates. qRT-PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE) and band sequencings were performed. The cultural method is able to detect less than 15% of what is evaluable with bio-molecular methods. A low sample alfa-diversity and a high beta-biodiversity in relation to feedstock and process were observed. Sequencing showed microorganisms with a hygienic-sanitary relevance such as , , , , and . Integrated cultural and biomolecular methods can be more exhaustive to evaluate bioaerosol's exposure in the occupational environment.
生物气溶胶暴露与废物的生物能源生产及其对职业和居住环境中人类健康的影响是目前研究的热点。与暴露相关的健康风险主要包括传染病、过敏、慢性支气管炎和阻塞性肺疾病。风险评估的关键点是生物气溶胶的质量和数量特征。本研究旨在评估不同方法在分析生物能源厂采集的生物气溶胶中的应用。本研究涉及意大利的六家处理生物质和能源生产的工厂。通过表面空气系统（SAS）采样器进行生物气溶胶的培养评估，并从 PM0.49 样品和低熔点琼脂板中提取 DNA。进行 qRT-PCR 后进行变性梯度凝胶电泳（DGGE）和条带测序。培养方法能够检测到的生物分子方法可检测到的不到 15%。与原料和工艺有关的样本α多样性较低，β生物多样性较高。测序显示了与卫生-卫生有关的微生物，如、、、、和。综合培养和生物分子方法可以更全面地评估职业环境中的生物气溶胶暴露。
