Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito universitario s/n, Campus Universitario # 2 31125, Chihuahua, Mexico.
Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito universitario s/n, Campus Universitario # 2 31125, Chihuahua, Mexico.
Carbohydr Polym. 2019 Oct 15;222:114998. doi: 10.1016/j.carbpol.2019.114998. Epub 2019 Jun 18.
The effects of pH (3.5, 4.5, and 5.5) and UV-C irradiation dose (12.8, 24.2, 35.8, and 54.6 mJ/cm) on the physicochemical properties changes in 10% Aloe vera gel blends; in addition, the acemannan concentration and structural changes in the precipitated polysaccharides were evaluated. A thermal treatment (TT; 45 s at 90 °C) was used for comparison. In contrast to TT, a dose of 24.2 mJ/cm did not induce significant changes of free sugar content. Moreover, TT and UV-C irradiation did not significantly affect the content of mannose but increased those of galactose, fructose, and glucose. H NMR analysis revealed minimal changes in the isolated fractions of acemannan, indicating that compared to the unprocessed control sample, the acemannan deacetylation was more pronounced by TT (27%) than by UV-C irradiation (11% at 54.6 mJ/cm), without any significant difference between the two. UV-C irradiation of Aloe vera gel blends at pH 3.5 and 24.2 mJ/cm was an alternative to TT and efficiently preserve the characteristics of acemannan.
pH 值(3.5、4.5 和 5.5)和 UV-C 辐照剂量(12.8、24.2、35.8 和 54.6 mJ/cm)对 10%库拉索芦荟凝胶混合物物理化学性质变化的影响;此外,评估了沉淀多糖中乙酰甘露聚糖的浓度和结构变化。热处理(TT;90°C 时 45 秒)用于比较。与 TT 相比,24.2 mJ/cm 的剂量不会引起游离糖含量的显著变化。此外,TT 和 UV-C 辐照不会显著影响甘露糖的含量,但会增加半乳糖、果糖和葡萄糖的含量。H NMR 分析显示分离的乙酰甘露聚糖部分变化很小,表明与未经处理的对照样品相比,TT(27%)比 UV-C 辐照(54.6 mJ/cm 时 11%)更能显著脱乙酰化乙酰甘露聚糖,两者之间没有显著差异。在 pH 值为 3.5 和 24.2 mJ/cm 时,对库拉索芦荟凝胶混合物进行 UV-C 辐照是 TT 的替代方法,可有效保持乙酰甘露聚糖的特性。