Dental Biomaterials Science Program, Graduate School, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Materials Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Carbohydr Polym. 2015 Nov 20;133:556-66. doi: 10.1016/j.carbpol.2015.07.039. Epub 2015 Jul 17.
Acemannan, an acetylated polymannose from Aloe vera, induces tissue repair. We investigated the role of acemannan's acetyl-groups on its physical and biological properties. Deacetylated acemannan (DeAcAM) was prepared and characterized. The physical properties and microscopic structure of DeAcAM were evaluated using water solubility, contact angle, X-ray diffraction, and scanning-electron microscopy. The activity of DeAcAM on cell proliferation and gene expression were assessed. Acemannan and DeAcAM structures were simulated and the acemannan tetramer diad and its completely deacetylated structure were also determined. Increased acemannan deacetylation reduced its water solubility and hydrophilicity. Complete deacetylation altered acemannan's conformation to a partial crystal structure. The bioactivity of acemannan was reduced corresponding to its deacetylation. Acemannan induced cell proliferation, and VEGF and Collagen I expression; however, 100% DeAcAM did not. The simulated structures of the acemannan diad and the completely deacetylated diad were different. We conclude acetyl-groups affect acemannan's structure and physical/biological properties.
乙酰化多聚糖Acemannan 是从芦荟中提取的，可诱导组织修复。我们研究了 Acemannan 乙酰基团对其物理和生物学特性的影响。制备并表征了脱乙酰 Acemannan（DeAcAM）。通过水溶性、接触角、X 射线衍射和扫描电子显微镜评估了 DeAcAM 的物理性质和微观结构。评估了 DeAcAM 对细胞增殖和基因表达的活性。模拟了 Acemannan 和 DeAcAM 的结构，并确定了 Acemannan 四聚体二联体及其完全脱乙酰结构。增加 Acemannan 的脱乙酰程度会降低其水溶性和亲水性。完全脱乙酰会改变 Acemannan 的构象为部分晶体结构。Acemannan 的生物活性与其脱乙酰程度相关。Acemannan 可诱导细胞增殖和 VEGF 和 Collagen I 的表达；然而，100%的 DeAcAM 则没有。Acemannan 二联体和完全脱乙酰二联体的模拟结构不同。我们得出结论，乙酰基团影响 Acemannan 的结构和物理/生物学特性。
