Mulaudzi-Masuku Takalani, Ikebudu Vivian, Muthevhuli Mpho, Faro Andrew, Gehring Christoph A, Iwuoha Emmanuel
Department of Biotechnology, University of the Western Cape, Bellville, South Africa.
Department of Chemistry, Biology & Biotechnology, University of Perugia, Perugia, Italy.
Bioinform Biol Insights. 2019 Jul 12;13:1177932219860813. doi: 10.1177/1177932219860813. eCollection 2019.
Heme oxygenases (HOs) have a major role in phytochrome chromophore biosynthesis, and chromophores in turn have anti-oxidant properties. Plant heme oxygenases are divided into the HO1 sub-family comprising HO1, HO3, and HO4, and the HO2 sub-family, which consists of 1 member, HO2. This study identified and characterized 4 heme oxygenase members from . Multiple sequence alignments showed that the heme oxygenase signature motif (QAFICHFYNI/V) is conserved across all HO proteins and that they share above 90% sequence identity with other cereals. Quantitative real-time polymerase chain reaction revealed that HO genes were expressed in leaves, stems, and roots, but most importantly their transcript level was induced by osmotic stress, indicating that they might play a role in stress responses. These findings will strengthen our understanding of the role of heme oxygenases in plant stress responses and may contribute to the development of stress tolerant crops.
血红素加氧酶（HOs）在光敏色素发色团生物合成中起主要作用，而发色团反过来具有抗氧化特性。植物血红素加氧酶分为包含HO1、HO3和HO4的HO1亚家族，以及由1个成员HO2组成的HO2亚家族。本研究从……中鉴定并表征了4个血红素加氧酶成员。多序列比对表明，血红素加氧酶特征基序（QAFICHFYNI/V）在所有HO蛋白中保守，并且它们与其他谷物的序列同一性超过90%。定量实时聚合酶链反应显示，HO基因在叶、茎和根中表达，但最重要的是其转录水平受渗透胁迫诱导，表明它们可能在胁迫应答中起作用。这些发现将加强我们对血红素加氧酶在植物胁迫应答中作用的理解，并可能有助于培育耐胁迫作物。
