Tepe Merve, Arslan Şevki, Koralay Tamer, Mercan Doğan Nazime
Department of Biology, Faculty of Science and Arts, Pamukkale University, Denizli, Turkey.
Department of Geology, Faculty of Engineering, Pamukkale University, Denizli, Turkey.
Turk J Biol. 2019 Jun 13;43(3):198-208. doi: 10.3906/biy-1901-56. eCollection 2019.
In the present study, the properties of calcium carbonate mineralization and urease and carbonic anhydrase activities of U17 isolated from calcareous soil of Denizli (Turkey) were analyzed. CaCO3 was produced in all growth phases. Strain U17 showed 0.615 ± 0.092 µmol/min/mg urease enzyme activity in calcium mineralization medium and 1.315 ± 0.021 µmol/min/mg urease enzyme activity in Luria-Bertani medium supplemented with urea, whereas it showed 36.03 ± 5.48 nmol/min/mg carbonic anhydrase enzyme activity in CaCO3 precipitation medium and 28.82 ± 3.31 nmol/min/mg carbonic anhydrase enzyme activity in Luria-Bertani medium supplemented with urea. The urease B protein expression level of strain U17 was detected by western blotting for the first time. The produced CaCO3 crystals were analyzed by X-ray diffraction, X-ray fluorescence, confocal RAMAN spectrophotometer, scanning electron microscopy, and electron probe microanalyzer for the evaluation of their morphological and elemental properties. Rhombohedral vaterite and layered calcite crystals were clearly detected and verified by mineralogical analyses. All these results showed that strain U17 can be used in many engineering and geological applications due to its CaCO3 precipitation ability.
在本研究中，分析了从土耳其代尼兹利钙质土壤中分离出的U17的碳酸钙矿化特性以及脲酶和碳酸酐酶活性。在所有生长阶段均产生了碳酸钙。菌株U17在钙矿化培养基中的脲酶酶活性为0.615±0.092 μmol/分钟/毫克，在添加尿素的Luria-Bertani培养基中的脲酶酶活性为1.315±0.021 μmol/分钟/毫克，而在碳酸钙沉淀培养基中的碳酸酐酶酶活性为36.03±5.48 nmol/分钟/毫克，在添加尿素的Luria-Bertani培养基中的碳酸酐酶酶活性为28.82±3.31 nmol/分钟/毫克。首次通过蛋白质免疫印迹法检测了菌株U17的脲酶B蛋白表达水平。通过X射线衍射、X射线荧光、共聚焦拉曼光谱仪、扫描电子显微镜和电子探针微分析仪对产生的碳酸钙晶体进行了分析，以评估其形态和元素特性。通过矿物学分析清楚地检测并验证了菱面体球霰石和层状方解石晶体。所有这些结果表明，菌株U17因其碳酸钙沉淀能力可用于许多工程和地质应用。
