• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-543 通过靶向 CYP3A5 在口腔鳞状细胞癌中发挥 novel oncogene 的作用。

miR‑543 acts as a novel oncogene in oral squamous cell carcinoma by targeting CYP3A5.

机构信息

Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.

出版信息

Oncol Rep. 2019 Sep;42(3):973-990. doi: 10.3892/or.2019.7230. Epub 2019 Jul 11.

DOI:10.3892/or.2019.7230
PMID:31322243
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6667884/
Abstract

MicroRNAs (miRNAs/miRs) are small non‑coding RNAs that can act as oncogenes or tumor‑suppressor genes in human cancer. Previous studies have revealed that abnormal expression of miRNAs is closely associated with tumor cell cycle, differentiation, growth and apoptosis. miR‑543 is expressed abnormally in a wide variety of cancers and has been associated with cellular proliferation, apoptosis, and invasion; however, the effect of miR‑543 remains unknown in oral squamous cell carcinoma (OSCC). In the present study, the expression level of miR‑543 in OSCC cell lines and tissues was investigated by RT‑qPCR. A series of experiments was then performed to elucidate the functions of miR‑543 in OSCC, such as CCK‑8 assay, colony formation assay, flow cytometry, cell cycle distribution assay and cell apoptosis assay and Transwell assay. miR‑543 expression was significantly upregulated in tumors from patients with OSCC and in OSCC cell lines. Overexpression of miR‑543 promoted the proliferation, invasion and migration of OSCC cell lines, and inhibited cell apoptosis. In addition, the present study identified cytochrome P450 family 3 subfamily A member 5 (CYP3A5) as a direct target of miR‑543 using software analysis and dual‑luciferase reporter assays. In conclusion, the results of the present study suggest that miR‑543 acts as a tumor promoter and serves a vital role in OSCC proliferation and invasion. These results confirm that miR‑543 may serve as a potential novel target for the treatment of OSCC.

摘要

微小 RNA（miRNA/miRs）是一种小的非编码 RNA，可在人类癌症中作为癌基因或肿瘤抑制基因发挥作用。先前的研究表明，miRNA 的异常表达与肿瘤细胞周期、分化、生长和凋亡密切相关。miR-543 在多种癌症中表达异常，与细胞增殖、凋亡和侵袭有关；然而，miR-543 在口腔鳞状细胞癌（OSCC）中的作用尚不清楚。在本研究中，通过 RT-qPCR 检测了 OSCC 细胞系和组织中 miR-543 的表达水平。然后进行了一系列实验，以阐明 miR-543 在 OSCC 中的功能，如 CCK-8 测定、集落形成测定、流式细胞术、细胞周期分布测定、细胞凋亡测定和 Transwell 测定。miR-543 在 OSCC 患者的肿瘤和 OSCC 细胞系中表达显著上调。miR-543 的过表达促进了 OSCC 细胞系的增殖、侵袭和迁移，并抑制了细胞凋亡。此外，本研究通过软件分析和双荧光素酶报告基因实验鉴定细胞色素 P450 家族 3 亚家族 A 成员 5（CYP3A5）为 miR-543 的直接靶标。综上所述，本研究结果表明，miR-543 作为一种肿瘤促进因子，在 OSCC 的增殖和侵袭中发挥着重要作用。这些结果证实，miR-543 可能成为治疗 OSCC 的一种新的潜在靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/870520e66ffe/or-42-03-0973-g14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/ef1756423100/or-42-03-0973-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/5e4c39537c54/or-42-03-0973-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/294eb2d2f0a4/or-42-03-0973-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/a107d92d6d5d/or-42-03-0973-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/be31d02d8ffe/or-42-03-0973-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/7b211612dc05/or-42-03-0973-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/34f61e1ab9bb/or-42-03-0973-g09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/be51982254b2/or-42-03-0973-g10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/0e5be98d1e56/or-42-03-0973-g11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/7dac4cbc3ef8/or-42-03-0973-g12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/a1442c896ebe/or-42-03-0973-g13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/870520e66ffe/or-42-03-0973-g14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/ef1756423100/or-42-03-0973-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/5e4c39537c54/or-42-03-0973-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/294eb2d2f0a4/or-42-03-0973-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/a107d92d6d5d/or-42-03-0973-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/be31d02d8ffe/or-42-03-0973-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/7b211612dc05/or-42-03-0973-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/34f61e1ab9bb/or-42-03-0973-g09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/be51982254b2/or-42-03-0973-g10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/0e5be98d1e56/or-42-03-0973-g11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/7dac4cbc3ef8/or-42-03-0973-g12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/a1442c896ebe/or-42-03-0973-g13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cbcc/6667884/870520e66ffe/or-42-03-0973-g14.jpg

相似文献

1
miR‑543 acts as a novel oncogene in oral squamous cell carcinoma by targeting CYP3A5.miR-543 通过靶向 CYP3A5 在口腔鳞状细胞癌中发挥 novel oncogene 的作用。
Oncol Rep. 2019 Sep;42(3):973-990. doi: 10.3892/or.2019.7230. Epub 2019 Jul 11.
2
MicroRNA‑199a‑5p suppresses migration and invasion in oral squamous cell carcinoma through inhibiting the EMT‑related transcription factor SOX4.微小 RNA-199a-5p 通过抑制 EMT 相关转录因子 SOX4 抑制口腔鳞状细胞癌的迁移和侵袭。
Int J Mol Med. 2019 Jul;44(1):185-195. doi: 10.3892/ijmm.2019.4174. Epub 2019 Apr 25.
3
Long noncoding RNA UCA1 promotes cell growth, migration, and invasion by targeting miR-143-3p in oral squamous cell carcinoma.长链非编码 RNA UCA1 通过靶向口腔鳞状细胞癌中的 miR-143-3p 促进细胞生长、迁移和侵袭。
Cancer Med. 2020 May;9(9):3115-3129. doi: 10.1002/cam4.2808. Epub 2020 Mar 4.
4
Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma.IFI27 基因敲低抑制口腔鳞状细胞癌细胞的增殖和侵袭。
World J Surg Oncol. 2018 Mar 27;16(1):64. doi: 10.1186/s12957-018-1371-0.
5
MicroRNA‑495 targets Notch1 to prohibit cell proliferation and invasion in oral squamous cell carcinoma.微小 RNA-495 通过靶向 Notch1 抑制口腔鳞状细胞癌的细胞增殖和侵袭。
Mol Med Rep. 2019 Jan;19(1):693-702. doi: 10.3892/mmr.2018.9616. Epub 2018 Nov 1.
6
Function of miR‑152 as tumor suppressor in oral squamous cell carcinoma cells by targeting c‑MET.miR-152 通过靶向 c-MET 在口腔鳞状细胞癌细胞中作为肿瘤抑制因子的功能。
Oncol Rep. 2018 Mar;39(3):1173-1180. doi: 10.3892/or.2017.6157. Epub 2017 Dec 15.
7
Molecular Pathogenesis of the Coronin Family: Facilitates Migration and Invasion Abilities in Oral Squamous Cell Carcinoma.冠状蛋白家族的分子发病机制：促进口腔鳞状细胞癌的迁移和侵袭能力。
Int J Mol Sci. 2021 Nov 24;22(23):12684. doi: 10.3390/ijms222312684.
8
Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.肿瘤细胞与 M2 型巨噬细胞之间的相互作用是 miR-550a-3-5p 下调介导的 HPV 阳性口咽鳞状细胞癌进展所必需的。
J Exp Clin Cancer Res. 2020 Jun 3;39(1):102. doi: 10.1186/s13046-020-01602-1.
9
Interplay between ΔNp63 and miR-138-5p regulates growth, metastasis and stemness of oral squamous cell carcinoma.ΔNp63与miR-138-5p之间的相互作用调节口腔鳞状细胞癌的生长、转移和干性。
Oncotarget. 2017 Mar 28;8(13):21954-21973. doi: 10.18632/oncotarget.15752.
10
MiR-376c-3p regulates the proliferation, invasion, migration, cell cycle and apoptosis of human oral squamous cancer cells by suppressing HOXB7.微小RNA-376c-3p通过抑制HOXB7调控人口腔鳞状癌细胞的增殖、侵袭、迁移、细胞周期及凋亡。
Biomed Pharmacother. 2017 Jul;91:517-525. doi: 10.1016/j.biopha.2017.04.050. Epub 2017 May 5.

引用本文的文献

1
The role of MicroRNAs in the diagnosis and treatment of oral premalignant disorders.MicroRNAs 在口腔癌前病变的诊断和治疗中的作用。
Odontology. 2024 Oct;112(4):1023-1032. doi: 10.1007/s10266-024-00934-0. Epub 2024 Apr 15.
2
The Role of CYP3A in Health and Disease.细胞色素P450 3A在健康与疾病中的作用
Biomedicines. 2022 Oct 24;10(11):2686. doi: 10.3390/biomedicines10112686.
3
Research progress and clinical application prospects of miRNAs in oral cancer.miRNAs 在口腔癌中的研究进展及临床应用前景。

本文引用的文献

1
miR-214 regulates papillary thyroid carcinoma cell proliferation and metastasis by targeting PSMD10.miR-214 通过靶向 PSMD10 调节甲状腺乳头状癌细胞的增殖和转移。
Int J Mol Med. 2018 Dec;42(6):3027-3036. doi: 10.3892/ijmm.2018.3902. Epub 2018 Sep 27.
2
miR-181b inhibits chemoresistance in cisplatin-resistant H446 small cell lung cancer cells by targeting Bcl-2.微小RNA-181b通过靶向Bcl-2抑制顺铂耐药的H446小细胞肺癌细胞的化疗耐药性。
Arch Med Sci. 2018 Jun;14(4):745-751. doi: 10.5114/aoms.2018.73131. Epub 2018 Feb 2.
3
Overexpression of ADAM9 in oral squamous cell carcinoma.
Mol Biol Rep. 2022 Nov;49(11):10653-10665. doi: 10.1007/s11033-022-07604-w. Epub 2022 Jun 20.
4
Potential lncRNA Biomarkers for HBV-Related Hepatocellular Carcinoma Diagnosis Revealed by Analysis on Coexpression Network.基于共表达网络分析筛选出的乙型肝炎病毒相关肝细胞癌潜在长链非编码 RNA 标志物
Biomed Res Int. 2021 Oct 15;2021:9972011. doi: 10.1155/2021/9972011. eCollection 2021.
5
Bioinformatics analysis of the expression and role of microRNA-221-3p in head and neck squamous cell carcinoma.miRNA-221-3p 在头颈部鳞状细胞癌中的表达及作用的生物信息学分析。
BMC Cancer. 2021 Apr 12;21(1):395. doi: 10.1186/s12885-021-08039-5.
6
MicroRNAs as Modulators of Oral Tumorigenesis-A Focused Review.微小RNA作为口腔肿瘤发生的调节因子——综述
Int J Mol Sci. 2021 Mar 4;22(5):2561. doi: 10.3390/ijms22052561.
7
LncRNA SNHG6 promotes breast cancer progression and epithelial-mesenchymal transition via miR-543/LAMC1 axis.长链非编码 RNA SNHG6 通过 miR-543/LAMC1 轴促进乳腺癌进展和上皮-间充质转化。
Breast Cancer Res Treat. 2021 Jul;188(1):1-14. doi: 10.1007/s10549-021-06190-y. Epub 2021 Mar 29.
8
MiR-503-5p functions as an oncogene in oral squamous cell carcinoma by targeting Smad7.miR-503-5p 通过靶向 Smad7 在口腔鳞状细胞癌中发挥癌基因作用。
Histol Histopathol. 2020 Aug;35(8):893-901. doi: 10.14670/HH-18-220. Epub 2020 Apr 22.
9
MicroRNA-96-5p promotes proliferation, invasion and EMT of oral carcinoma cells by directly targeting FOXF2.miR-96-5p 通过直接靶向 FOXF2 促进口腔癌细胞的增殖、侵袭和 EMT。
Biol Open. 2020 Mar 11;9(3):bio049478. doi: 10.1242/bio.049478.
ADAM9在口腔鳞状细胞癌中的过表达。
Oncol Lett. 2018 Jan;15(1):495-502. doi: 10.3892/ol.2017.7284. Epub 2017 Oct 30.
4
miR-20a Regulates FAS Expression in Osteosarcoma Cells by Modulating Promoter Activity and Can be Therapeutically Targeted to Inhibit Lung Metastases.miR-20a 通过调节启动子活性调控骨肉瘤细胞中 FAS 的表达，可作为治疗靶点抑制肺转移。
Mol Cancer Ther. 2018 Jan;17(1):130-139. doi: 10.1158/1535-7163.MCT-17-0042. Epub 2017 Oct 27.
5
miR-543 promotes colorectal cancer proliferation and metastasis by targeting .微小RNA-543通过靶向……促进结直肠癌的增殖和转移。
Oncotarget. 2017 Jul 22;8(35):59246-59256. doi: 10.18632/oncotarget.19495. eCollection 2017 Aug 29.
6
UALCAN: A Portal for Facilitating Tumor Subgroup Gene Expression and Survival Analyses.UALCAN：一个促进肿瘤亚组基因表达和生存分析的平台。
Neoplasia. 2017 Aug;19(8):649-658. doi: 10.1016/j.neo.2017.05.002. Epub 2017 Jul 18.
7
The differential regulation of microRNAs is associated with oral cancer.微小RNA的差异调节与口腔癌相关。
Oncol Rep. 2017 Sep;38(3):1613-1620. doi: 10.3892/or.2017.5811. Epub 2017 Jul 12.
8
TRAIL, Wnt, Sonic Hedgehog, TGFβ, and miRNA Signalings Are Potential Targets for Oral Cancer Therapy.肿瘤坏死因子相关凋亡诱导配体（TRAIL）、Wnt、音猬因子、转化生长因子β（TGFβ）和微小RNA（miRNA）信号通路是口腔癌治疗的潜在靶点。
Int J Mol Sci. 2017 Jul 14;18(7):1523. doi: 10.3390/ijms18071523.
9
Association of NR1I2, CYP3A5 and ABCB1 genetic polymorphisms with variability of temsirolimus pharmacokinetics and toxicity in patients with metastatic bladder cancer.NR1I2、CYP3A5和ABCB1基因多态性与转移性膀胱癌患者替西罗莫司药代动力学及毒性变异性的相关性
Cancer Chemother Pharmacol. 2017 Sep;80(3):653-659. doi: 10.1007/s00280-017-3379-5. Epub 2017 Jul 4.
10
miR-543 functions as a tumor suppressor in glioma in vitro and in vivo.miR-543在体外和体内的胶质瘤中发挥肿瘤抑制作用。
Oncol Rep. 2017 Aug;38(2):725-734. doi: 10.3892/or.2017.5712. Epub 2017 Jun 12.