College of Animal Science, Anhui Science and Technology University, No. 9, Donghua Road, 233100 Fengyang City, Anhui, China.
Folia Morphol (Warsz). 2020;79(2):303-310. doi: 10.5603/FM.a2019.0082. Epub 2019 Jul 19.
Ten healthy adult Huang-huai white goats were selected and sacrificed by jugular vein bleeding after anaesthesia to observe the distribution characteristics of the histological structure of the intestinal mucosa, goblet cells, IgA, and CD3+ lymphocytes.
Three sections of the duodenum, the jejunum, and the ileum were immediately collected and fixed with 4% paraformaldehyde for 72 h to prepare tissue sections. After haematoxylin and eosin, periodic acid Schiff, and immunohistochemical staining was performed, the distribution characteristics of goblet cells, IgA-positive cells, and CD3+ lymphocytes were observed. Results showed high columnar epithelial cells in the duodenum and jejunum of Huang-huai white goat and low columnar epithelial cells in the ileum mucosa.
Mucopolysaccharides secreted by intestinal goblet cells were mainly neutral, and the number of ileum goblet cells was significantly higher than that of the duodenum and the jejunum (p < 0.05). IgA-positive cells were distributed in the lamina propria of the duodenum, and the number of cells was significantly higher than that in the jejunum and the ileum (p < 0.01). The significant difference was found between the jejunum and the ileum (p < 0.01). The CD3+ cells in the intestinal mucosa were distributed in the lamina propria mucosae, and some of the positive cells in the jejunum were distributed between epithelial cells. CD3+ cells had the largest number in the jejunal lamina propria but had the lowest number in the ileum.
The jejunum was significantly higher than the duodenum (p < 0.05), and the ileum was much less than the jejunum (p < 0.01).
选择 10 只健康成年槐山羊，通过颈静脉放血麻醉后处死，观察其空肠、回肠和十二指肠黏膜组织学结构、杯状细胞、IgA、CD3+淋巴细胞的分布特点。
立即采集 3 段十二指肠、空肠和回肠组织，用 4%多聚甲醛固定 72 h 后制作组织切片，分别行苏木精-伊红（HE）、过碘酸雪夫（PAS）和免疫组织化学染色，观察杯状细胞、IgA 阳性细胞和 CD3+淋巴细胞的分布特点。结果表明，槐山羊空肠和十二指肠黏膜有高柱状上皮细胞，回肠黏膜有低柱状上皮细胞。
肠黏膜杯状细胞分泌的黏多糖主要为中性，回肠杯状细胞数量明显高于十二指肠和空肠（p<0.05）。IgA 阳性细胞分布在十二指肠固有层，细胞数量明显高于空肠和回肠（p<0.01），空肠和回肠之间差异有统计学意义（p<0.01）。CD3+细胞在黏膜固有层内分布，部分空肠阳性细胞分布在细胞间，其中空肠固有层内 CD3+细胞数量最多，回肠内最少。
空肠显著高于十二指肠（p<0.05），回肠显著低于空肠（p<0.01）。
