Department of Biotechnology, Indian Institute of Technology Roorkee, 247667, India.
Department of Biotechnology, Indian Institute of Technology Roorkee, 247667, India.
Res Vet Sci. 2019 Aug;125:244-252. doi: 10.1016/j.rvsc.2019.07.001. Epub 2019 Jul 9.
Bovine milk contains different components with nutritional and immunological benefits. It is easily accessible and a rich source of potential markers reflective of pathophysiological conditions; however, little is known about the changes in protein abundance associated with variation across breeds and seasons. In this study, we performed a comprehensive proteomic profiling of whey proteins from Holstein Friesian cow and Murrah buffalo across summer and winter seasons. Collectively, 490 proteins were identified with 113 and 144 differentially expressed proteins across seasons in cow and buffalo, respectively. Breed specific proteins like secretoglobin, e-cadherin and cathepsin-L were detected exclusively in HF, while basigin, conglutinin, and thrombomodulin were identified exclusively in Mu. Acute phase proteins (e.g. haptoglobin and α1AG) were more abundant in summer while antimicrobial proteins (e.g. conglutinin and osteopontin) were upregulated in winter. Similarly, proteins involved in lipid homeostasis (e.g. perilipin 2 and acyl CoA binding protein) showed breed specific variations. Selected representative mass spectrometric proteins (e.g. gelsolin and osteopontin) were validated by Western blot analysis. Results of this study indicate the dynamic nature of milk protein and provide a foundation for future studies of whey proteins which may be linked to diseases specific across breeds and seasons.
牛乳含有具有营养和免疫益处的不同成分。它易于获取，是反映生理病理状态的潜在标志物的丰富来源；然而，关于与品种和季节变化相关的蛋白质丰度变化知之甚少。在这项研究中，我们对荷斯坦-弗里森奶牛和印度奶水牛的乳清蛋白进行了全面的蛋白质组学分析，分别在夏季和冬季进行了分析。共鉴定出 490 种蛋白质，牛和水牛在季节间分别有 113 种和 144 种差异表达蛋白。在 HF 中检测到乳球蛋白、E-钙黏蛋白和组织蛋白酶-L 等品种特异性蛋白，而在 Mu 中检测到 basigin、conglutinin 和血栓调节蛋白等蛋白。在夏季，急性期蛋白（如触珠蛋白和α1AG）更为丰富，而在冬季，抗菌蛋白（如 conglutinin 和骨桥蛋白）则上调。同样，参与脂质稳态的蛋白（如 perilipin 2 和酰基辅酶 A 结合蛋白）也表现出品种特异性变化。通过 Western blot 分析验证了选定的代表性质谱蛋白（如凝溶胶蛋白和骨桥蛋白）。本研究结果表明了乳蛋白的动态性质，并为未来针对与品种和季节相关的疾病的乳清蛋白研究提供了基础。
