Department of Genetic Biochemistry, Faculty of Biotechnology, Wroclaw University, Przybyszewskiego 63, 51-148, Wroclaw, Poland; Linum Foundation, Wroclaw, Poland.
Department of Genetic Biochemistry, Faculty of Biotechnology, Wroclaw University, Przybyszewskiego 63, 51-148, Wroclaw, Poland.
Plant Physiol Biochem. 2019 Sep;142:234-245. doi: 10.1016/j.plaphy.2019.07.009. Epub 2019 Jul 5.
Previous studies showed that chalcone synthase (chs) silencing in flax (Linum usitatisimum) induces a signal transduction cascade that leads to extensive modification of plant metabolism. Result presented in the current study, performed on field grown flax plants - (across the whole vegetation period) demonstrates that, in addition to its role in tannin and lignin biosynthesis, the chs gene also participates in the regulation of flavone biosynthesis during plant growth. Apigenin and luteolin glycosides constitute the flavones, the major group of flavonoids in flax. Alterations in their levels correlate with plant growth, peaking at the flower initiation stage. Suppression of chs gene expression causes significant changes in the ratio of flavone constituents at the early stage of flax growth. A significant correlation between flavonoid 3'-hydroxylase (F3'H) gene expression and accumulation of luteolin glycosides has been found, indicating that flavone biosynthesis during flax growth and development is regulated by temporal expression of this gene. The lack of such a correlation between the flavone synthase (FNS) gene and flavone accumulation in the course of plant growth suggests that the main route of flavone biosynthesis is mediated by eriodictyol. This is the first report indicating the ratio of flavone constituents as a potent marker of flax growth stages and temporal expression of F3'H, the key gene of their biosynthesis.
先前的研究表明，在亚麻（Linum usitatisimum）中沉默查尔酮合酶（chs）会诱导信号转导级联反应，从而导致植物代谢的广泛修饰。本研究在田间生长的亚麻植物（整个生长期间）上进行，结果表明，chs 基因除了在单宁和木质素生物合成中起作用外，还参与了植物生长过程中类黄酮生物合成的调节。芹黄素和木犀草素糖苷构成了类黄酮，是亚麻中黄酮类化合物的主要组分之一。它们的水平变化与植物生长相关，在花起始阶段达到峰值。chs 基因表达的抑制会导致在亚麻生长的早期阶段类黄酮成分的比例发生显著变化。发现类黄酮 3'-羟化酶（F3'H）基因表达与木犀草素糖苷的积累之间存在显著相关性，表明在亚麻生长和发育过程中类黄酮生物合成受该基因的时间表达调控。在植物生长过程中，黄酮合酶（FNS）基因与黄酮积累之间缺乏这种相关性表明，类黄酮生物合成的主要途径是通过桃叶珊瑚苷介导的。这是第一个表明类黄酮成分的比例作为亚麻生长阶段的有效标志物的报告，以及 F3'H 的时间表达，这是它们生物合成的关键基因。
