Pfaller M, Davenport D, Bale M, Barrett M, Koontz F, Massanari R M
Veterans Administration Medical Center, Iowa City, Iowa 52240.
Eur J Clin Microbiol Infect Dis. 1988 Feb;7(1):30-3. doi: 10.1007/BF01962167.
The macro-test for slime production by coagulase-negative staphylococci was adapted to a spectrophotometric micro-test assay to obtain more objective and quantitative information on slime production. A total of 135 isolates of coagulase-negative staphylococci (70 macro-test-positive and 65 macro-test-negative) were tested by both methods. The quantitative micro-test optical density readings were (mean +/- SD) 1.176 +/- 0.294 and 0.130 +/- 0.095 for the macro-test-positive and -negative groups, respectively. The micro-test was reproducible and demonstrated quantitative differences in slime production among the different species of coagulase-negative staphylococci. Aside from Staphylococcus epidermidis, the majority of the coagulase-negative staphylococci had very low optical density readings, indicating little or no slime production under the conditions employed in this assay. This test allows one to study the relative production of slime by different strains and species of coagulase-negative staphylococci, and may be useful in studying the effects of different conditions, such as antibiotic exposure, on slime production.
用于检测凝固酶阴性葡萄球菌产生黏液的宏观试验被改编为分光光度法微观试验，以获取关于黏液产生的更客观和定量的信息。两种方法对总共135株凝固酶阴性葡萄球菌（70株宏观试验阳性和65株宏观试验阴性）进行了检测。宏观试验阳性和阴性组的定量微观试验光密度读数分别为（平均值±标准差）1.176±0.294和0.130±0.095。微观试验具有可重复性，并显示出不同种凝固酶阴性葡萄球菌在黏液产生方面的定量差异。除表皮葡萄球菌外，大多数凝固酶阴性葡萄球菌的光密度读数非常低，表明在本试验所用条件下几乎不产生或不产生黏液。该试验可用于研究不同菌株和种的凝固酶阴性葡萄球菌的相对黏液产生情况，并且可能有助于研究不同条件（如抗生素暴露）对黏液产生的影响。
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