Department of Neuroscience and Center for Translational Research in Neurodegenerative Disease, University of Florida, Gainesville, FL 32601, USA.
Int J Mol Sci. 2019 Jul 18;20(14):3524. doi: 10.3390/ijms20143524.
Impairments in translation have been increasingly implicated in the pathogenesis and progression of multiple neurodegenerative diseases. Assessing the spatiotemporal dynamics of translation in the context of disease is a major challenge. Recent developments in proteomic analyses have enabled the resolution of nascent peptides in a short timescale on the order of minutes. In addition, a quantitative analysis of translation has progressed in vivo, showing remarkable potential for coupling these techniques with cognitive and behavioral outcomes. Here, we review these modern approaches to measure changes in translation and ribosomal function with a specific focus on current applications in the mammalian brain and in the study of neurodegenerative diseases.
翻译障碍越来越多地与多种神经退行性疾病的发病机制和进展有关。评估疾病背景下翻译的时空动态是一个主要挑战。蛋白质组学分析的最新进展使人们能够在几分钟的短时间尺度内解析新生肽。此外，体内翻译的定量分析也取得了进展，显示出将这些技术与认知和行为结果相结合的巨大潜力。在这里，我们回顾了这些现代方法来测量翻译和核糖体功能的变化，特别关注当前在哺乳动物大脑中的应用以及神经退行性疾病的研究。
