Division Gastroenterology-Hepatology, Department of Internal Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, 6202 AZ Maastricht, The Netherlands.
Top Institute Food and Nutrition, 6700 AN Wageningen, The Netherlands.
Nutrients. 2019 Jul 9;11(7):1554. doi: 10.3390/nu11071554.
Intestinal barrier function is suggested to decrease with aging and may be improved by pectin intake. The aim of this study was to investigate the effects of four weeks pectin supplementation on gastrointestinal barrier function in vivo and ex vivo in different age groups. In a randomized, double-blind, placebo-controlled, parallel study, 52 healthy young adults (18-40 years) and 48 healthy elderly (65-75 years) received 15 g/day pectin or placebo for four weeks. Pre- and post-intervention, in vivo gastrointestinal permeability by a multisugar test, and defense capacity in mucosal samples were assessed. Sigmoid biopsies were collected post-intervention from subgroups for Ussing chamber experiments and gene transcription of barrier-related genes. Pectin intervention did not affect in vivo gastroduodenal, small intestinal, colonic, and whole gut permeability in young adults nor in elderly ( ≥ 0.130). Salivary and fecal sIgA and serum IgA were not significantly different between pectin versus placebo in both age groups ( ≥ 0.128). In both young adults and elderly, no differences in transepithelial electrical resistance and fluorescein flux ( ≥ 0.164) and relative expression of genes analyzed ( ≥ 0.222) were found between pectin versus placebo. In conclusion, intestinal barrier function was not affected by four weeks pectin supplementation neither in healthy young adults nor in healthy elderly.
肠道屏障功能随年龄增长而下降，而果胶摄入可能会改善这种情况。本研究旨在调查四周果胶补充对不同年龄组体内和体外胃肠道屏障功能的影响。在一项随机、双盲、安慰剂对照、平行研究中，52 名健康的年轻人（18-40 岁）和 48 名健康的老年人（65-75 岁）每天接受 15 克果胶或安慰剂补充剂，为期四周。在干预前后，通过多聚糖测试评估体内胃肠道通透性，以及黏膜样本的防御能力。干预后，从小部分参与者中采集乙状结肠活检样本，用于 Ussing 室实验和与屏障相关基因的转录。果胶干预并未影响年轻人和老年人的体内胃十二指肠、小肠、结肠和整个肠道通透性（≥0.130）。果胶与安慰剂相比，两组的唾液和粪便 sIgA 和血清 IgA 均无显著差异（≥0.128）。在年轻人和老年人中，果胶与安慰剂相比，跨上皮电阻和荧光素通量（≥0.164）以及分析的基因相对表达（≥0.222）均无差异。总之，四周的果胶补充并未影响健康的年轻人和健康的老年人的肠道屏障功能。
