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从南极碘杆菌中分离和鉴定紫色杆菌素:一种非致病性耐寒微生物。

Isolation and characterization of violacein from an Antarctic Iodobacter: a non-pathogenic psychrotolerant microorganism.

机构信息

Fundación Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago, Chile.

Departamento de Ingeniería Química, Universidad de Santiago de Chile, Alameda 3363, Estación Central, Santiago, Chile.

出版信息

Extremophiles. 2020 Jan;24(1):43-52. doi: 10.1007/s00792-019-01111-w. Epub 2019 Jul 19.

Abstract

Violacein is an intensely purple pigment synthesized by various genera of bacteria that has been discovered to have a wide range of interesting biological activities which range from anticarcinogenic to antibacterial. One of the hindrances for its real-life application is that the first microorganisms found to produce the compound may act as opportunistic pathogens. Here, we report the isolation and characterization of violacein from a non-pathogenic Antarctic Iodobacter strain. Its anti-microbial properties were also tested. The method proposed here for the purification of violacein shows high yields, indicating that this Antarctic microorganism could be a valuable source for this important pigment. This is the first characterization of violacein from an Antarctic Iodobacter strain and here we also present a viable method to obtain this pigment for potential biotechnological applications.

摘要

紫灰菌素是一种由多种细菌属合成的深紫色色素,现已发现其具有广泛的有趣的生物活性,从抗癌到抗菌。其实际应用的一个障碍是,最初发现产生该化合物的微生物可能是机会性病原体。在这里,我们报告了一种非致病性南极碘杆菌中紫灰菌素的分离和特性。还测试了其抗菌特性。这里提出的紫灰菌素的纯化方法显示出高产量,表明这种南极微生物可能是这种重要色素的有价值的来源。这是首次对南极碘杆菌菌株中的紫灰菌素进行表征,我们还提出了一种可行的方法来获得这种色素,以用于潜在的生物技术应用。

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