Kumagai Hiroshi, Makihara Takeshi, Funayama Toru, Sato Kosuke, Noguchi Hiroshi, Abe Tetsuya, Koda Masao, Yamazaki Masashi
Department of Orthopaedic Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
J Artif Organs. 2019 Dec;22(4):294-299. doi: 10.1007/s10047-019-01120-8. Epub 2019 Jul 19.
Affinos (Kuraray Co., Ltd., Tokyo, Japan) is a beta-tricalcium phosphate (TCP) artificial bone comprising a novel unidirectional porous structure with 57% porosity. This study examined angiogenesis and bone formation over time with unidirectional porous beta-TCP (UDPTCP). Ten Japanese White rabbits were used in this study. A 5 × 8-mm rectangular area of periosteum was resected, followed by preparation of a cortical bone defect using a high-speed bur. UDPTCP was embedded in the defect in the direction of the pores, parallel to the axis of the tibia. Tissue samples were harvested at 2 weeks (n = 3) and 6 weeks (n = 7) after implantation. Just before euthanasia, the vasculature of the lower limb was perfused with saline from the femoral artery and filled with MICROFIL (Flow Tech, Inc., Carver, MA) to create a vascular cast. The tibia was cut longitudinally at the center of the material. Decalcified sagittal sections treated with hematoxylin and eosin staining, undecalcified sagittal sections treated with Villanueva-Goldner staining, and axial unstained sections were used for histological evaluation. The lengths of the largest vessels and newly formed bone at the material border were measured in a sagittal section. Both lengths were significantly larger at 6 weeks than at 2 weeks. In the axial sections at 2 weeks, newly formed vessels filled with blue dye grew along the pores of the UDPTCP. Mature bone tissue with a lamellar structure was observed at 6 weeks. Our histological findings demonstrated that angiogenesis and bone formation occur over time in UDPTCP.
Affinos（日本东京可乐丽株式会社）是一种β-磷酸三钙（TCP）人工骨，具有新型单向多孔结构，孔隙率为57%。本研究考察了单向多孔β-TCP（UDPTCP）随时间的血管生成和骨形成情况。本研究使用了10只日本白兔。切除5×8毫米的矩形骨膜区域，然后用高速牙钻制备皮质骨缺损。将UDPTCP沿孔隙方向平行于胫骨轴线嵌入缺损处。在植入后2周（n = 3）和6周（n = 7）采集组织样本。在安乐死前不久，从股动脉向下肢血管灌注生理盐水，并用MICROFIL（美国马萨诸塞州卡弗市Flow Tech公司）填充以制作血管铸型。在材料中心纵向切开胫骨。用苏木精和伊红染色处理的脱钙矢状切片、用Villanueva-Goldner染色处理的未脱钙矢状切片以及轴向未染色切片用于组织学评估。在矢状切片中测量材料边界处最大血管和新形成骨的长度。这两个长度在6周时均显著大于2周时。在2周时的轴向切片中，充满蓝色染料的新形成血管沿UDPTCP的孔隙生长。在6周时观察到具有板层结构的成熟骨组织。我们的组织学研究结果表明，UDPTCP中血管生成和骨形成随时间发生。
